LOCUS       sequence-285294-        4922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2639
FEATURES             Location/Qualifiers
     source          1..4922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2014..2023
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3051..3068)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 accgccacca tgtattcggc ccacaggccc ctgatgcccg cgtccagcgc ggcctcccgt
       61 ggcctcggca tgttcgtgtg gacgaatgtg gaacctcgct ctgtggctgt gttcccttgg
      121 cactccttag tccccttcct ggcacccagc cagcctgacc cctccgtgca gccgagcgag
      181 gcccagcaac ctgccagcca cccagtggcc tccaaccaga gcaaagaacc tgctgagtcg
      241 gcagctgttg ctcatgaacg gccaccaggt gggacaggga gtgctgaccc tgagcggccc
      301 cctggagcca catgccctga gagcccagga cccggacccc cacacccttt gggggtggtg
      361 gaatctggta agggtccgcc tcccaccacg gaggaggagg cctccggccc cccaggagag
      421 ccccggctgg acagtgagac agagagtgac catgatgatg ccttcctctc catcatgtct
      481 cctgagatcc agttgcctct accgcccgga aaacgtcgga cccagtccct cagtgcccta
      541 cccaaggaac gggactcatc ttctgagaag gatggacgca gccccaacaa gcgggagaag
      601 gaccacatcc ggcggcccat gaatgccttc atgatcttca gcaagcggca ccgggccctg
      661 gtccaccagc gtcatcccaa ccaggacaac cggaccgtca gcaagatcct gggcgagtgg
      721 tggtatgccc tggggcccaa ggagaagcag aagtaccacg acctggcctt ccaggtgaag
      781 gaggcccact tcaaggccca cccagattgg aagtggtgca acaaggaccg aaagaagtcc
      841 agctcagagg ccaagcccac gagcctgggg ctggcaggag ggcacaagga gacgcgggag
      901 cggagcatgt cggagacggg cactgctgct gcccctgggg tgtcctctga gctcctgtcc
      961 gttgcagccc agacactcct gagctcagac accaaggctc cggggagcag ctcctgtggg
     1021 gcagaacggc tacacacagt tgggggacct ggctcagccc ggccccgagc tttctcccac
     1081 agcggggtac acagcctgga cggcggagaa gtagacagtc aggcgctaca ggaactgacg
     1141 cagatggtgt ctggccctgc atcgtactct ggcccaaagc cttctaccca gtatggagct
     1201 ccaggaccct ttgcagcccc tggtgaggga ggtgccttgg cggccactgg gcggcccccg
     1261 ctgctgccca cccgagcttc tcgttctcag cgtgcggcca gtgaggacat gacgagtgat
     1321 gaggagcgca tggtcatctg tgaggaggaa ggggatgatg atgtcattgc tgacgatggc
     1381 ttcggcacca ctgacattga tctcaagtgc aaggagcggg tgaccgacag cgagagtggg
     1441 gacagctctg gggaggaccc agagggcaac aagggctttg gtcggaaggt gttttcacct
     1501 gtgatccgtt cctcctttac ccactgccgc cccccactgg accctgagcc cccagggccc
     1561 ccggatcctc ctgtagcctt tggcaaaggc tatggttccg ccccatcctc ctctgcgtcc
     1621 tcgcctgctt cctcctcagc ctcggcagcc acctccttct cactgggctc aggaaccttc
     1681 aaggcccagg agtctggtca gggcagcaca gcgggccccc tacggccccc accccctggg
     1741 gctgggggtc cagcgacacc ttccaaggca acccggttcc tcccaatgga tcctgccacc
     1801 ttccggcgca agagacccga aagtgtgggt ggcctggagc caccaggccc ctcagtcatc
     1861 gcggcccctc ccagcggagg aggaaacatc ctgcagacac tggtgctgcc cccaaacaag
     1921 gaggagcaag agggcggcgg agccagagtg ccctccgccc ccgccccatc actggcctat
     1981 ggggccccag cagctcccct gtcccgtcct gccgccacca tggtcaccaa tgtggtgcgg
     2041 cctgtcagca gcactcctgt gcccatcgcc tctaagccct tccccacctc tggccgggct
     2101 gaggcgtctc caaatgacac agcaggtgcc aggactgaaa tgggcactgg gtctcgggtg
     2161 cctgggggct ccccgctggg tgtcagctta gtgtattcgg acaagaagtc ggcagcagcc
     2221 acctcaccag ccccacactt ggtggctgga cccctgctgg gcactgtggg gaaggcgcct
     2281 gccactgtca ctaacctact ggtgggcacc ccggggtatg gggcccctgc gccccctgct
     2341 gtccagttca ttgcccaggg ggcccctggt ggtgggacca ctgcgggctc aggagcaggt
     2401 gctgggagtg gccccaatgg gccagtaccc ctgggcatcc tgcaaccagg tgccctgggc
     2461 aaggctgggg gaatcaccca ggtacagtac atcctgccca cgctgcccca gcagcttcag
     2521 gtggcacctg ccccagcacc agcccctggg accaaggcag cggctcccag cggccctgca
     2581 cccaccacca gcatccgttt caccctccca ccgggcactt ccaccaacgg caaagtcctg
     2641 gctgccactg cacccactcc tggcatcccc atcctgcagt ctgtaccctc cgccccaccc
     2701 cccaaagccc agtcagtttc tcccgtgcag gccccgcccc cgggtggctc agcccagctg
     2761 ctgcctggga aggtcctagt gcctctggcc gcccctagca tgtcagtgcg gggtggaggg
     2821 gccggccagc cactgccact ggtgagcccg cccttctcag tacctgtgca gaatggtgcc
     2881 cagcccccca gcaagatcat ccagctgacc ccggtgcctg tgagcacacc cagcggcctg
     2941 gtgccgcccc tgagcccagc cacactccct ggacccacct ctcagcctca gaaggtcctg
     3001 ttgccctcct ccaccagaat cacctatgtg cagtcagcgg gcgggcacgc gctgcccctg
     3061 ggtaccagcc ctgcgtccag ccaggctgga acagtcacct cgtacgggcc cacgagctct
     3121 gtagctctag gcttcacctc gctggggccc agcggccccg ccttcgtgca gcccctgctc
     3181 tcaggccaag ccccactgct ggctcccggt caggtgggcg tgtcacctgt gcccagtccc
     3241 cagctgccgc ctgcctgtgc agcccccgga ggtcctgtca taacagcatt ttactctggc
     3301 agccctgcac ccacctcctc agcacccctg gcccagccat cccaggcccc cccaagcctg
     3361 gtctacactg tggccaccag cacaacccca cctgcagcca ccattctgcc caagggcccg
     3421 ccagcccctg ccactgccac cccagccccg actagccctt tccccagcgc cacaggttcc
     3481 atgacctaca gcttagtggc ccccaaggcc cagcggccca gcccgaaggc cccccagaaa
     3541 gtgaaggcag ccatcgccag cattcccgtg gggtcctttg aggcaggtgc ctctgggcgg
     3601 cctggccctg caccccggca gcctctggag cctggcccag tccgagagcc aactgcccca
     3661 gagtctgagc ttgaggggca gcccacacca ccagcccctc cacccctgcc agagacctgg
     3721 actcccacgg cccggagcag ccccccactg cccccacctg ctgaggagcg gaccagcgcc
     3781 aagggccctg agaccatggc cagcaaattc cccagctcat cttcagactg gcgcgtccct
     3841 gggcagggcc tggagaatcg tggggagcct cccactcctc ccagcccggc cccagctcca
     3901 gctgtagccc ctggtggcag cagcgagagc agcagtgggc gggcagccgg ggacaccccg
     3961 gagcgcaagg aggcggctgg tactggcaag aaggtgaagg tgcggccccc gcccctgaag
     4021 aagacctttg actctgtgga caacagggtc ctgtcagaag tggacttcga agagcgcttt
     4081 gctgagttgc ctgagtttcg gcctgaggag gtgctgccct cccccaccct gcagtctctg
     4141 gccacctcac cccgggccat cctgggctct taccgcaaga agaggaagaa ctccacggac
     4201 ctggattcag cacccgagga ccccacctcg cccaagcgca agatgagaag acgctccagc
     4261 tgcagctcgg agcccaacac ccccaagagt gccaagtgcg agggggacat cttcaccttt
     4321 gaccgtacag gtacagaagc cgaggacgtg cttggggagc tagagtatga caaggtgcca
     4381 tactcctccc tgcggcgcac cctggaccag cgccgggccc tggtcatgca gctctttcag
     4441 gaccatggct tcttcccgtc agcccaggcc acagccgcct tccaggcccg ctatgcagac
     4501 atctttccct ccaaggtttg tctgcagttg aagatccgtg aggtgcgcca gaagatcatg
     4561 caggctgcca ctcccacgga gcagccccct ggagctgagg ctcctctccc tgtaccgccc
     4621 cccactggca ccgctgctgc ccctgccccc actcccagcc ccgcaggggg ccctgacccc
     4681 acctcaccca gctcggactc tggcacggcc caggctgccc cgccactgcc tccaccccca
     4741 gagtcggggc ctggacagcc tggctgggag ggggctcccc agccctcccc cccaccccca
     4801 ggtccctcca cagctgccac aggcaggtag taatgaacta gtccgtaact tgaaagtatt
     4861 tcgatttctt ggctttatat atcttgtgga aaggacgatt tctgttcgct gtaggtcaag
     4921 ca
//
