LOCUS       sequence-285348-         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2935
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(509..533)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             541..582
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgacgactaa tcccaaaccg
       61 aataaggcat taaaggtcaa gaaggaggcg ggcgagaacg ccccggtgct cagcgatgat
      121 gagctggtgt ccatgtcggt gcgggagctg aaccagcacc tgcggggtct caccaaggag
      181 gaggtgaccc gcctgaagca gcgtcggcgc acactcaaga accgcggcta cgcggccagc
      241 tgccgcatca agcgggtgac gcagaaggag gagctggagc ggcagcgcgt ggagctgcag
      301 caggaggtgg agaagctggc gcgtgagaac agcagcatgc ggctggagct ggacgccctg
      361 cgctccaagt acgaggcgct gcagaccttc gcgcgcaccg tggcccgggg acctgtggcg
      421 ccctccaagg tggccaccac cagcgtcatc accatcgtca agtccaccga gctctcctcc
      481 acctccgtgc ccttctcggc tgcatccttg ccaactttct tgtacaaagt ggttgatatc
      541 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
      601 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgagacagat
      661 aataccattg tccccgt
//
