LOCUS       sequence-285354-        1352 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2937
FEATURES             Location/Qualifiers
     source          1..1352
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1184..1208)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1216..1257
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tgatgttctc gggcttcaac
       61 gcagactacg aggcgtcatc ctcccgctgc agcagcgcgt ccccggccgg ggatagcctc
      121 tcttactacc actcacccgc agactccttc tccagcatgg gctcgcctgt caacgcgcag
      181 gacttctgca cggacctggc cgtctccagt gccaacttca ttcccacggt cactgccatc
      241 tcgaccagtc cggacctgca gtggctggtg cagcccgccc tcgtctcctc tgtggcccca
      301 tcgcagacca gagcccctca ccctttcgga gtccccgccc cctccgctgg ggcttactcc
      361 agggctggcg ttgtgaagac catgacagga ggccgagcgc agagcattgg caggaggggc
      421 aaggtggaac agttatctcc agaagaagaa gagaaaagga gaatccgaag ggaaaggaat
      481 aagatggctg cagccaaatg ccgcaaccgg aggagggagc tgactgatac actccaagcg
      541 gagacagacc aactagaaga tgagaagtct gctttgcaga ccgagattgc caacctgctg
      601 aaggagaagg aaaaactaga gttcatcctg gcagctcacc gacctgcctg caagatccct
      661 gatgacctgg gcttcccaga agagatgtct gtggcttccc ttgatctgac tgggggcctg
      721 ccagaggttg ccaccccgga gtctgaggag gccttcaccc tgcctctcct caatgaccct
      781 gagcccaagc cctcagtgga acctgtcaag agcatcagca gcatggagct gaagaccgag
      841 ccctttgatg acttcctgtt cccagcatca tccaggccca gtggctctga gacagcccgc
      901 tccgtgccag acatggacct atctgggtcc ttctatgcag cagactggga gcctctgcac
      961 agtggctccc tggggatggg gcccatggcc acagagctgg agcccctgtg cactccggtg
     1021 gtcacctgta ctcccagctg cactgcttac acgtcttcct tcgtcttcac ctaccccgag
     1081 gctgactcct tccccagctg tgcagctgcc caccgcaagg gcagcagcag caatgagcct
     1141 tcctctgact cgctcagctc acccacgctg ctggccctgt agaacccagc tttcttgtac
     1201 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1261 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1321 aaggacgagg accgaagata atctcacctt at
//
