LOCUS       sequence-285402-        2516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2953
FEATURES             Location/Qualifiers
     source          1..2516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2348..2372)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2380..2421
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt tcccgcagag ccggcacccg
       61 acgccgcacc aggctgcagg ccagcccttc aagttcacta tcccggagtc cctggaccgg
      121 attaaagagg aattccagtt cctgcaggcg cagtatcaca gccttaaatt ggaatgtgag
      181 aaactggcaa gtgaaaagac agaaatgcag aggcactatg tgatgtatta tgaaatgtca
      241 tatggattaa acattgaaat gcacaaacag actgaaatcg ccaagagatt gaatacgatt
      301 tgtgcacaag tcatcccatt tctgtctcag gaacatcaac aacaggtggc ccaggctgtt
      361 gaacgtgcca aacaggtgac catggcagag ttgaatgcca tcatcgggca gcagcagttg
      421 caagctcagc atctttctca tggccacgga cccccagttc cccttacgcc tcacccttcg
      481 ggacttcagc ctcctggaat cccgcccctc gggggcagtg ccggccttct tgcgctgtct
      541 agtgctctga gtgggcagtc tcacttggca ataaaagatg acaagaagca ccacgatgca
      601 gagcaccaca gagacagaga gccgggcaca agtaattccc tcctggtccc agacagtcta
      661 agaggcacag ataaacgcag aaatggacct gaattttcca atgacatcaa gaaaaggaag
      721 gtggatgata aggactccag ccactatgac agtgatggtg acaaaagcga tgacaactta
      781 gttgtggatg tgtctaatga ggacccttct tctccgcgag caagccctgc ccactcgccc
      841 cgggaaaatg gaatcgacaa aaatcgcctg ctaaagaagg atgcttctag cagtccagct
      901 tccacggcct cctcggcaag ttccacttct ttgaaatcca aagaaatgag cttgcatgaa
      961 aaagccagca cgcctgttct gaaatccagc acaccaacgc ctcggagcga catgccaacg
     1021 ccgggcacca gcgccactcc aggcctccgt ccaggtctcg gcaagcctcc agccatagac
     1081 cccctcgtta accaagcggc agctggcttg aggacacccc tggcagtgcc cggcccatat
     1141 cctgctcctt ttgggatggt cccccacgct ggcatgaacg gcgagctgac cagcccaggc
     1201 gctgcctacg ccagtttaca caacatgtcg ccccagatga gcgccgcagc cgccgcggcc
     1261 gccgtggtgg cctacgggcg ctcccccatg gtggggtttg atcctccccc tcacatgaga
     1321 gtacctacca ttcctccaaa cctggcagga atccctgggg ggaaacctgc atactccttc
     1381 cacgttactg cagacggtca gatgcagcct gtcccttttc cccccgacgc cctcatcgga
     1441 cccggaatcc cccggcatgc tcgccagatc aacaccctca accacgggga ggtggtgtgc
     1501 gctgtgacca tcagcaaccc cacgagacac gtgtacacag gcgggaaggg ctgcgtcaag
     1561 gtctgggaca tcagccaccc tggcaataag agccctgtct cccagctcga ctgtctgaac
     1621 agagacaatt atatccgttc ctgtaaattg ctacccgatg gctgcactct catagtggga
     1681 ggggaagcca gtactttgtc catttgggac ctggcggctc caaccccgcg catcaaggcg
     1741 gagctgacgt cctcggcccc cgcctgctac gccctggcca tcagccccga ttccaaggtc
     1801 tgcttctcat gctgcagcga cggcaacatc gctgtgtggg atctgcacaa ccagacacta
     1861 gtgaggcaat tccagggcca cacagacgga gccagctgta ttgacatttc taatgatggc
     1921 accaagctct ggacgggtgg tttggacaac acagtcaggt cctgggacct gcgcgagggg
     1981 cggcagctgc agcagcacga cttcacctcc cagatcttct ccctggggta ctgccccacc
     2041 ggggagtggc tggcagtggg catggagagc agcaatgtgg aggtgctgca cgtgaacaag
     2101 cctgacaagt accagctgca cctgcatgag agctgcgtgc tgtccctgaa atttgcttac
     2161 tgtggtaaat ggtttgtgag tactggaaaa gataacctcc tcaatgcttg gcggaccccc
     2221 tatggagcca gcatattcca gtccaaagag tcctcgtcag tgcttagctg tgacatctct
     2281 gtggatgata agtacatagt cactggctcg ggggacaaga aggctacagt ctatgaagtc
     2341 atctactgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     2401 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     2461 tcttggcttt atatatcttg tggaaaggac gagttgagta gatatgcccg aggggc
//
