LOCUS       sequence-285450-        1556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2969
FEATURES             Location/Qualifiers
     source          1..1556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1054..1065)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1388..1412)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1420..1461
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc ctcacaactc catcagatct
       61 ggccatggag ggctgaacca gctgggaggg gcctttgtga atggcagacc tctgccggaa
      121 gtggtccgcc agcgcatcgt agacctggcc caccagggtg taaggccctg cgacatctct
      181 cgccagctcc gcgtcagcca tggctgcgtc agcaagatcc ttggcaggta ctacgagact
      241 ggcagcatcc ggcctggagt gatagggggc tccaagccca aggtggccac ccccaaggtg
      301 gtggagaaga ttggggacta caaacgccag aaccctacca tgtttgcctg ggagatccga
      361 gaccggctcc tggctgaggg cgtctgtgac aatgacactg tgcccagtgt cagctccatt
      421 aatagaatca tccggaccaa agtgcagcaa ccattcaacc tccctatgga cagctgcgtg
      481 gccaccaagt ccctgagtcc cggacacacg ctgatcccca gctcagctgt aactcccccg
      541 gagtcacccc agtcggattc cctgggctcc acctactcca tcaatgggct cctgggcatc
      601 gctcagcctg gcagcgacaa gaggaaaatg gatgacagtg atcaggatag ctgccgacta
      661 agcattgact cacagagcag cagcagcgga ccccgaaagc accttcgcac ggatgccttc
      721 agccagcacc acctcgagcc gctcgagtgc ccatttgagc ggcagcacta cccagaggcc
      781 tatgcctccc ccagccacac caaaggcgag cagggcctct acccgctgcc cttgctcaac
      841 agcaccctgg acgacgggaa ggccaccctg accccttcca acacgccact ggggcgcaac
      901 ctctcgactc accagaccta ccccgtggtg gcagatcctc actcaccctt cgccataaag
      961 caggaaaccc ccgaggtgtc cagttctagc tccacccctt cctctttatc tagctccgcc
     1021 tttttggatc tgcagcaagt cggctccggg gtcccgccct tcaatgcctt tccccatgct
     1081 gcctccgtgt acgggcagtt cacgggccag gccctcctct cagggcgaga gatggtgggg
     1141 cccacgctgc ccggataccc accccacatc cccaccagcg gacagggcag ctatgcctcc
     1201 tctgccatcg caggcatggt ggcaggaagt gaatactctg gcaatgccta tggccacacc
     1261 ccctactcct cctacagcga ggcctggcgc ttccccaact ccagcttgct gagttcccca
     1321 tattattaca gttccacatc aaggccgagt gcaccgccca ccactgccac ggcctttgac
     1381 catctgtacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1441 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1501 tcttggcttt atatatcttg tggaaaggac gaagcacctc aacccactct tggtcg
//
