LOCUS       sequence-285489-        2270 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2982
FEATURES             Location/Qualifiers
     source          1..2270
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2102..2126)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2134..2175
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ctcggaaatt agtgatgttc
       61 agggatgttg ccattgactt ctctcaggaa gagtgggaat gcctggactc tgctcagaga
      121 gatttgtata gagatgtgat gttggagaac tatagcaact tggtatcact agacttgcct
      181 tcaaggtgtg caagtaagga cttatctcca gaaaagaaca cttatgaaac agaattatcc
      241 caatgggaaa tgagtgacag acttgaaaac tgtgatcttg aagagtccaa ttccagggat
      301 tatttggaag ccaaaggcaa gatggagaag caacaagaaa atcagaagga atatttcagg
      361 caagggatga tcatatatga caaaatgtcc attttcaacc agcatactta cttatctcaa
      421 cattcaagat gtcattctac tgagaaaccc tataaatgta aggaatgtgg gaaagccttc
      481 agacgagcct cacacctaac acaacatcaa agtattcata ctggtgaaaa accctatgaa
      541 tgtaagcaat gcgggaaggc ctttagtcgt gattcacaac tcagtcttca tcagagactt
      601 catactggtg agaaacccta tgcatgtaag gaatgtggga aggcctttac tcaaagctca
      661 caacttattt tacatcatag aattcatact ggtgaaaaac catataaatg tgaagaatgt
      721 gggaaagcct ttattcgtag ctcacaactt acccgacatc aaaaagttca tactggtgag
      781 aaaccttatg aatgtaaaga atgtgggaag gcctttactc agaattcaca acttacacta
      841 caccagagac ttcatactgg tgaaaagctc tatgaatgta aagaatgtag gaaggtcttt
      901 actcagctct cacaacttat tctgcataag agaattcata ccggtgagaa accctatgaa
      961 tgtaaggaat gtggaaaagc ttttatttgt ggctcacagc tttctcaaca tcagaaaatt
     1021 cataatgggg aaaaaccata tgaatgtaag gaatgtggaa gggcctttat tcggggctca
     1081 ctactgatgc aacatcagag gattcatact ggtgaaaaac cctataaatg tgaagaatgt
     1141 gggaaggcct ttatccgtgg ctcacaactt actcaacacc agagaattca caccaatgaa
     1201 aagccctatg aatgtaagga atgtggaaag atgtttagtc atggctcaca acttactcaa
     1261 catcagagaa tacacactgg tgagaaaccc tatcaatgta aggaatgtgg aaaagcgttt
     1321 aatcgtggct cactccttac acgacaccag aggattcata ctggtgagaa accctatgaa
     1381 tgtaaagaat gtggaaaaac ctttagtcgt ggctcagaac ttactcaaca tgagcgaatt
     1441 cacacaggtg agaaacccta tgaatgtaag gaatgtggga aatcttttat tcgtggttcc
     1501 cagcttactc aacatcagag aatccatact ggtgagaaac cttatgaatg taaagaatgt
     1561 agaatggcct ttactcagag ttcacatctt tcccaacatc aaagacttca cactggtgag
     1621 aaaccctatg tgtgtaatga atgtggaaag gcctttgcgc gtggcttact acttatacaa
     1681 catcagagaa ttcatactgg tgagaaacca tatcaatgta aggaatgtgg gaaagccttt
     1741 attcgtggtt cacagttgac tcaacatcag cgaattcaca ctggagaaaa accctatgaa
     1801 tgcaaggagt gtggcaaggc ctttagtcat ggctctcagc ttactctaca tcagagaatc
     1861 catactggtg agaagcccta tgaatgcaga gaatgtagaa aggcctttac tcagagttca
     1921 catctttctc ggcatcagag aattcatact ggtgagaaac catatcaatg taaggaatgt
     1981 gggaaggcct ttactcgtgg ttcacagcta actcaacatc agagaattca tatcagtgag
     2041 aaatcttttg aatataagga atgtgggatt gactttagtc atggctcaca agtttacatg
     2101 tgcccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     2161 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     2221 ctttatatat cttgtggaaa ggacgaagcg gaccgagcaa agctactggt
//
