LOCUS       sequence-285588-        1631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3015
FEATURES             Location/Qualifiers
     source          1..1631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1463..1487)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1495..1536
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcttcc aattgaccat ggtggacacg
       61 gaaagcccac tctgccccct ctccccactc gaggccggcg atctagagag cccgttatct
      121 gaagagttcc tgcaagaaat gggaaacatc caagagattt cgcaatccat cggcgaggat
      181 agttctggaa gctttggctt tacggaatac cagtatttag gaagctgtcc tggctcagat
      241 ggctcggtca tcacggacac gctttcacca gcttcgagcc cctcctcggt gacttatcct
      301 gtggtccccg gcagcgtgga cgagtctccc agtggagcat tgaacatcga atgtagaatc
      361 tgcggggaca aggcctcagg ctatcattac ggagtccacg cgtgtgaagg ctgcaagggc
      421 ttctttcggc gaacgattcg actcaagctg gtgtatgaca agtgcgaccg cagctgcaag
      481 atccagaaaa agaacagaaa caaatgccag tattgtcgat ttcacaagtg cctttctgtc
      541 gggatgtcac acaacgcgat tcgttttgga cgaatgccaa gatctgagaa agcaaaactg
      601 aaagcagaaa ttcttacctg tgaacatgac atagaagatt ctgaaactgc agatctcaaa
      661 tctctggcca agagaatcta cgaggcctac ttgaagaact tcaacatgaa caaggtcaaa
      721 gcccgggtca tcctctcagg aaaggccagt aacaatccac cttttgtcat acatgatatg
      781 gagacactgt gtatggctga gaagacgctg gtggccaagc tggtggccaa tggcatccag
      841 aacaaggagg cggaggtccg catctttcac tgctgccagt gcacgtcagt ggagaccgtc
      901 acggagctca cggaattcgc caaggccatc ccaggcttcg caaacttgga cctgaacgat
      961 caagtgacat tgctaaaata cggagtttat gaggccatat tcgccatgct gtcttctgtg
     1021 atgaacaaag acgggatgct ggtagcgtat ggaaatgggt ttataactcg tgaattccta
     1081 aaaagcctaa ggaaaccgtt ctgtgatatc atggaaccca agtttgattt tgccatgaag
     1141 ttcaatgcac tggaactgga tgacagtgat atctcccttt ttgtggctgc tatcatttgc
     1201 tgtggagatc gtcctggcct tctaaacgta ggacacattg aaaaaatgca ggagggtatt
     1261 gtacatgtgc tcagactcca cctgcagagc aaccacccgg acgatatctt tctcttccca
     1321 aaacttcttc aaaaaatggc agacctccgg cagctggtga cggagcatgc gcagctggtg
     1381 cagatcatca agaagacgga gtcggatgct gcgctgcacc cgctactgca ggagatctac
     1441 agggacatgt actgagctag cgacccagct ttcttgtaca aagtggttga tatcggtaag
     1501 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1561 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgatca ccaaaaacac
     1621 atcgtctcat c
//
