LOCUS       sequence-285603-        1763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3020
FEATURES             Location/Qualifiers
     source          1..1763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1595..1619)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1627..1668
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcggccct tcccggcacg
       61 gtaccgagaa tgatgcggcc ggctccgggg cagaactacc cccgcacggg attccctttg
      121 gaagtgtcca ccccgcttgg ccaaggccgg gtcaatcagc tgggaggggt cttcatcaat
      181 gggcgacccc tgcctaacca catccgccac aagatagtgg agatggccca ccatggcatc
      241 cggccctgtg tcatctcccg acagctgcgt gtctcccacg gctgcgtctc caagattctt
      301 tgccgctacc aggagaccgg gtccatccgg cctggggcca tcggcggcag caagcccaga
      361 caggtggcga ctccggatgt agagaaaaag attgaggagt acaagaggga aaacccaggc
      421 atgttcagct gggagatccg ggacaggctg ctgaaggatg ggcactgtga ccgaagcact
      481 gtgccctcag tgagttcgat tagccgcgtg ctcagaatca agttcgggaa gaaagaggag
      541 gaggatgaag cggacaagaa ggaggacgac ggcgaaaaga aggccaaaca cagcatcgac
      601 ggcatcctgg gcgacaaagg gaaccggctg gacgagggct cggatgtgga gtcggaacct
      661 gacctcccac tgaagcgcaa gcagcgacgc agtcggacca cattcacggc cgagcagctg
      721 gaggagctgg agaaggcctt tgagaggacc cactacccag acatatacac ccgcgaggag
      781 ctggcgcaga ggaccaagct gacagaggcg cgtgtgcagg tctggttcag taaccgccgc
      841 gcccgttggc gtaagcaggc aggagccaac cagctggcgg cgttcaacca ccttctgcca
      901 ggaggcttcc cgcccaccgg catgcccacg ctgcccccct accagctgcc ggactccacc
      961 taccccacca ccaccatctc ccaagatggg ggcagcactg tgcaccggcc tcagcccctg
     1021 ccaccgtcca ccatgcacca gggcgggctg gctgcagcgg ctgcagccgc cgacaccagc
     1081 tctgcctacg gagcccgcca cagcttctcc agctactctg acagcttcat gaatccggcg
     1141 gcgccctcca accacatgaa cccggtcagc aacggcctgt ctcctcaggt gatgagcatc
     1201 ttgggcaacc ccagtgcggt gcccccgcag ccacaggctg acttctccat ctccccgctg
     1261 catggcggcc tggactcggc cacctccatc tcagccagct gcagccagcg ggccgactcc
     1321 atcaagccag gagacagcct gcccacctcc caggcctact gcccacccac ctacagcacc
     1381 accggctaca gcgtggaccc cgtggccggc tatcagtacg gccagtacgg ccagagtgag
     1441 tgcctggtgc cctgggcgtc ccccgtcccc attccttctc ccacccccag ggcctcctgc
     1501 ttgtttatgg agagctacaa ggtggtgtca gggtggggaa tgtccatttc acagatggaa
     1561 aaattgaagt ccagccagat ggaacagttc accttgccaa ctttcttgta caaagtggtt
     1621 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1681 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     1741 tgatgcccta ctataaccct tac
//
