LOCUS       sequence-285627-        1391 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3028
FEATURES             Location/Qualifiers
     source          1..1391
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1223..1247)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1255..1296
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tggctgcaaa agtggttcct
       61 atgcccccaa agccaaagca gtcctttata ctgagagttc cgccagactc caagctgggc
      121 caagacctac ttcgagatgc cactaacggg cccaagacca tccaccagct agtgctggag
      181 cacttcctca ccttcttgcc caagccaagc ctggtccagc ccagtcagaa agtcaaggag
      241 accttggtta ttatgaaaga tgtgagctca agccttcaga acagagtgca tcctcgtccc
      301 ttggtgaagc ttctgcccaa aggagtccaa aaggaacaag agacagtgtc tctgtatttg
      361 aaagctaacc ctgaggagct ggtggtcttt gaggatttga atgtatttca ctgccaggaa
      421 gaatgtgtga gcttggatcc tactcaacaa ctcacgtcag agaaggaaga tgacagcagt
      481 gtcggggaaa tgatgttact ggcagtcaat ggcagtaatc ctgaaggtga agatcctgag
      541 agggaacctg tagaaaatga agattataga gaaaagtctt cagatgatga tgaaatggat
      601 tcttccttgg tctctcagca gcctcccgat aaccaggaaa aggaacgact aaatacatcc
      661 attccacaaa aaaggaaaat gagaaatctg ttagttacca ttgagaatga tactcctcta
      721 gaggaactct caaaatatgt agacatcagt attattgccc ttactcgaaa tcggaggaca
      781 aggagatggt acacttgtcc actgtgtggg aaacagttta atgaaagttc ttacctcatt
      841 tcccaccaga ggacccacac tggagaaaaa ccctatgact gtaatcactg tgggaaaagc
      901 ttcaatcata aaacaaacct caataaacat gagcgaattc atacaggaga gaaaccttat
      961 tcctgttctc agtgtggaaa aaacttccgt cagaattctc atcggagtcg tcatgaagga
     1021 atccatataa gggagaagat atttaagtgt ccagaatgtg ggaaaacctt cccaaagaat
     1081 gaggagtttg tgcttcatct gcagagtcat gaggctgaga gaccatatgg ttgcaaaaaa
     1141 tgtgggagaa gatttggtcg gctgtcaaac tgtacccggc atgagaaaac ccactcagcc
     1201 tgtaagaccc gaaagcagaa gtacccaact ttcttgtaca aagtggttga tatcggtaag
     1261 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1321 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgactc tgcaatcatc
     1381 atatcgttac c
//
