LOCUS       sequence-285636-        1256 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3031
FEATURES             Location/Qualifiers
     source          1..1256
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1088..1112)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1120..1161
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg caatgagaca gacgccgctc
       61 acctgctctg gccacacgcg acccgtggtt gatttggcct tcagtggcat cacgccttat
      121 gggtatttct taatcagcgc ttgcaaagat ggtaaaccta tgctacgcca gggagataca
      181 ggagactgga ttggaacatt tttgggtcat aaaggtgctg tttggggtgc aacactgaat
      241 aaggatgcca ccaaagcagc tacagcagct gcagatttca cagccaaagt gtgggatgct
      301 gtctcaggag atgaattgat gaccctggct cataaacaca ttgtcaagac tgtggatttc
      361 acgcaggata gtaattattt gttaaccggg ggacaggata aactgttacg catatatgac
      421 ttgaacaaac ctgaagcaga acctaaggaa attagtggtc atacttctgg tataaaaaaa
      481 gctctgtggt gcagtgagga taaacagatt ctttctgctg atgacaaaac tgttcgactt
      541 tgggatcatg ctactatgac agaagtgaaa tctctaaatt ttaatatgtc tgttagtagt
      601 atggaatata ttcctgaggg agagattttg gttataactt atggacgatc tattgctttt
      661 catagtgcag taagtttgga cccaattaaa tcctttgaag ctcctgcaac catcaattct
      721 gcatctcttc atcctgagaa agaatttctt gttgcaggcg gtgaagattt taaactttat
      781 aagtatgatt ataatagtgg agaagaatta gaatcctaca agggacactt tggtcctatt
      841 cactgtgtga gatttagtcc tgatggagaa ctctatgcca gtggttcaga agatggaaca
      901 ttgagactat ggcaaactgt ggtaggaaaa acgtatggcc tttggaaatg tgtgcttcct
      961 gaagaagata gtggtgagct ggcaaagcca aagattggtt ttccagagac aacagaagag
     1021 gagctagaag aaattgcttc agagaattca gattgcatct ttccttcagc tcctgatgtt
     1081 aaggcctgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1141 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1201 tcttggcttt atatatcttg tggaaaggac gatgccatgc ggctgggatg gctatc
//
