LOCUS       sequence-285687-        2207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3048
FEATURES             Location/Qualifiers
     source          1..2207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2039..2063)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2071..2112
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga aaatgcatgc tgagaagaag
       61 cacaaatgta gtaagtgcag caattcgtac ggtacagaat gggacctgaa aagacatgca
      121 gaggactgtg gcaagacctt ccggtgcaca tgcggctgtc cctacgccag tagaacagca
      181 ctgcagtctc acatctaccg aactgggcac gagatacctg cagaacacag ggacccacct
      241 agtaagaaaa ggaaaatgga aaactgtgca caaaaccaga agttatccaa caagaccatt
      301 gaatcattga acaaccaacc aatccctaga ccagacactc aagaactaga agcttcagaa
      361 ataaagctag aaccatcttt tgaagactct tgtggctcta acactgacaa gcagactctt
      421 acaacaccac cgaggtatcc tcagaagttg cttttaccaa agcccaaagt ggctttggtt
      481 aaactacccg tgatgcagtt ttctgtcatg cctgtctttg tgcctacagc cgactcctca
      541 gcccagcctg tggtgttagg tgttgatcag ggctctgcca caggggctgt gcacttaatg
      601 cccttgtcag taggaaccct gatcctcggc ctagattcag aggcttgctc tcttaaggag
      661 agcctacctc ttttcaaaat tgctaatcct attgctggtg agccaataag tactggtgtt
      721 caagtgaact ttggtaaaag tccatctaat cctttacaag aactagggaa cacgtgtcaa
      781 aagaatagca tttcttcaat caacgtgcag acagatctgt cttatgcctc acaaaacttt
      841 ataccttctg cacagtgggc cactgctgat tcctctgtgt cgtcttgttc tcaaactgat
      901 ttgtcgtttg attctcaagt gtctcttccc attagtgttc acactcagac atttttgccc
      961 agctctaagg taacttcatc tatagctgct cagactgatg catttatgga cacctgtttc
     1021 cagtcaggtg gggtctccag agaaactcaa accagtggga tagaaagtcc aacggatgac
     1081 catgtacaga tggaccaagc tggaatgtgc ggagacattt ttgagagtgt tcattcatca
     1141 tataatgttg ctacaggtaa cattataagc aacagtttag tagcagagac agtaactcat
     1201 agtttgttac ctcagaatga gcctaagact ttaaatcaag atattgagaa atctgcacca
     1261 attataaatt tcagtgcaca gaatagtatg cttccttcac agaacatgac agataatcag
     1321 acccaaacca tagatttatt aagtgatttg gaaaacatct tgtcaagtaa tctgcctgcc
     1381 cagacattgg atcatcgtag tcttttgtct gacacaaatc ctggacctga cacccagctc
     1441 ccatctggcc cagcccagaa ccccggaatc gattttgata tcgaagagtt cttttcggcc
     1501 tcaaatatcc agactcaaac tgaagagagt gaacttagca ccatgaccac cgagccagtc
     1561 ttggagtcac tggacataga gactcaaacg gacttcttac tcgcagatac ctctgctcag
     1621 tcctatgggt gtaggggaaa ttctaacttc ttaggccttg agatgtttga cacacagaca
     1681 cagacagact taaacttttt cttagacagt agccctcatc tgcctctggg aagtattctg
     1741 aaacactcca gcttttccgt gagtactgat tcatctgaca cagagaccca aactgaagga
     1801 gtctccactg ctaaaaatat acctgctcta gaaagcaaag ttcagttgaa cagtacagaa
     1861 acacagacca tgagttctgg gtttgaaacc ctggggagct tgttcttcac cagcaacgaa
     1921 actcagacag caatggatga ctttcttctg gctgatctgg cctggaacac gatggagtct
     1981 cagttcagct ctgtagaaac ccagacttct gcggaaccac acacagtctc caacttctac
     2041 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     2101 ctcgattcta cgtagtaatg aactagcccg taacttgaaa gtatttcgat ttcttggctt
     2161 tatatatctt gtggaaagga cgatgtacct tcatcgatgc attatct
//
