LOCUS       sequence-285705-        3368 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3054
FEATURES             Location/Qualifiers
     source          1..3368
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3200..3224)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3232..3273
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtcgaggcg caagcaggcg
       61 aaaccccagc acatcaactc ggaggaggac cagggcgagc agcagccgca gcagcagacc
      121 ccggagtttg cagatgcggc cccagcggcg cccgcggcgg gggagctggg tgctccagtg
      181 aaccacccag ggaatgacga ggtggcgagt gaggatgaag ccacagtaaa gcggcttcgt
      241 cgggaggaga cgcacgtctg tgagaaatgc tgtgcggagt tcttcagcat ctctgagttc
      301 ctggaacata agaaaaattg cactaaaaat ccacctgtcc tcatcatgaa tgacagcgag
      361 gggcctgtgc cttcagaaga cttctccgga gctgtactga gccaccagcc caccagtccc
      421 ggcagtaagg actgtcacag ggagaatggc ggcagctcag aggacatgaa ggagaagccg
      481 gatgcggagt ctgtggtgta cctaaagaca gagacagccc tgccacccac cccccaggac
      541 ataagctatt tagccaaagg caaagtggcc aacactaacg tgaccttgca ggcactacgg
      601 ggcaccaagg tggcggtgaa tcagcggagc gcggatgcac tccctgcccc cgtgcctggt
      661 gccaacagca tcccgtgggt cctcgagcag atcttgtgtc tgcagcagca gcagctacag
      721 cagatccagc tcaccgagca gatccgcatc caggtgaaca tgtgggcctc ccacgccctc
      781 cactcaagcg gggcaggggc cgacactctg aagaccttgg gcagccacat gtctcagcag
      841 gtttctgcag ctgtggcttt gctcagccag aaagctggaa gccaaggtct gtctctggat
      901 gccttgaaac aagccaagct acctcacgcc aacatccctt ctgccaccag ctccctgtcc
      961 ccagggctgg cacccttcac tctgaagccg gatgggaccc gggtgctccc gaacgtcatg
     1021 tcccgcctcc cgagcgcttt gcttcctcag gccccgggct cggtgctctt ccagagccct
     1081 ttctccactg tggcactaga cacatccaag aaagggaagg ggaagccacc gaacatctcc
     1141 gcggtggatg tcaaacccaa agacgaggcg gccctctaca agcacaagtg taagtactgt
     1201 agcaaggttt ttgggactga tagctccttg cagatccacc tccgctccca cactggagag
     1261 agacccttcg tgtgctctgt ctgtggtcat cgcttcacca ccaagggcaa cctcaaggtg
     1321 cactttcacc gacatcccca ggtgaaggca aacccccagc tgtttgccga gttccaggac
     1381 aaagtggcgg ccggcaatgg catcccctat gcactctctg tacctgaccc catagatgaa
     1441 ccgagtcttt ctttagacag caaacctgtc cttgtaacca cctctgtagg gctacctcag
     1501 aatctttctt cggggactaa tcccaaggac ctcacgggtg gctccttgcc cggtgacctg
     1561 cagcctgggc cttctccaga aagtgagggt ggacccacac tccctggggt gggaccaaac
     1621 tataattccc caagggctgg tggcttccaa gggagtggga cccctgagcc agggtcagag
     1681 accctgaaat tgcagcagtt ggtggagaac attgacaagg ccaccactga tcccaacgaa
     1741 tgtctcattt gccaccgagt cttaagctgt cagagctccc tcaagatgca ttatcgcacc
     1801 cacaccgggg agagaccgtt ccagtgtaag atctgtggcc gagccttttc taccaaaggt
     1861 aacctgaaga cacaccttgg ggttcaccga accaacacat ccattaagac gcagcattcg
     1921 tgccccatct gccagaagaa gttcactaat gccgtgatgc tgcagcaaca tattcggatg
     1981 cacatgggcg gtcagattcc caacacgccc ctgccagaga atccctgtga ctttacgggt
     2041 tctgagccaa tgaccgtggg tgagaacggc agcaccggcg ctatctgcca tgatgatgtc
     2101 atcgaaagca tcgatgtaga ggaagtcagc tcccaggagg ctcccagcag ctcctccaag
     2161 gtccccacgc ctcttcccag catccactcg gcatcaccca cgctagggtt tgccatgatg
     2221 gcttccttag atgccccagg gaaagtgggt cctgcccctt ttaacctgca gcgccagggc
     2281 agcagagaaa acggttccgt ggagagcgat ggcttgacca acgactcatc ctcgctgatg
     2341 ggagaccagg agtatcagag ccgaagccca gatatcctgg aaaccacatc cttccaggca
     2401 ctctccccgg ccaatagtca agccgaaagc atcaagtcaa agtctcccga tgctgggagc
     2461 aaagcagaga gctccgagaa cagccgcact gagatggaag gtcggagcag tctcccttcc
     2521 acgtttatcc gagccccgcc gacctatgtc aaggttgaag ttcctggcac atttgtggga
     2581 ccctcgacat tgtccccagg gatgacccct ttgttagcag cccagccacg ccgacaggcc
     2641 aagcaacatg gctgcacacg gtgtgggaag aacttctcct ctgctagcgc tcttcagatc
     2701 cacgagcgga ctcacactgg agagaagcct tttgtgtgca acatttgtgg gcgagctttt
     2761 accaccaaag gcaacttaaa ggttcactac atgacacacg gggcgaacaa taactcagcc
     2821 cgccgtggaa ggaagttggc catcgagaac accatggctc tgttaggtac ggacggaaaa
     2881 agagtctcag aaatctttcc caaggaaatc ctggcccctt cagtgaatgt ggaccctgtt
     2941 gtgtggaacc agtacaccag catgctcaat ggcggtctgg ccgtgaagac caatgagatc
     3001 tctgtgatcc agagtggggg ggttcctacc ctcccggttt ccttgggggc cacctccgtt
     3061 gtgaataacg ccactgtctc caagatggat ggctcccagt cgggtatcag tgcagatgtg
     3121 gaaaaaccaa gtgctactga cggcgttccc aaacaccagt ttcctcactt cctggaagaa
     3181 aacaagattg cggtcagctt gccaactttc ttgtacaaag tggttgatat cggtaagcct
     3241 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     3301 agtatttcga tttcttggct ttatatatct tgtggaaagg acgacggacc gacacgagca
     3361 cacataat
//
