LOCUS       sequence-285729-        2024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3062
FEATURES             Location/Qualifiers
     source          1..2024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgaggc agcgctagtg attacgccgc agattcccat ggtaacagaa
       61 gagtttgtga aaccatcaca gggccatgtg acctttgagg atattgctgt gtacttctcc
      121 caggaggagt ggggcctcct tgatgaagct caaaggtgcc tgtatcatga tgtgatgctg
      181 gagaactttt cgcttatggc ctcagtaggt tgtttgcatg gaatagaggc tgaggaggcc
      241 ccttctgagc agactctttc tgcgcaagga gtgtcacagg ccaggactcc aaagctaggt
      301 ccttccatcc caaatgctca ttcttgtgag atgtgtatcc tggtcatgaa agacattttg
      361 tacctcagtg agcatcaggg gacacttccc tggcagaaac cttatacgtc tgtggccagt
      421 gggaaatggt tttcatttgg ttctaacctg caacagcacc agaaccagga cagtggagag
      481 aaacacatca gaaaggagga gagcagtgcc ttgcttctga atagctgcaa aattcctctg
      541 tcagacaatc ttttcccatg caaagatgtt gagaaggatt ttccaaccat cctgggcctt
      601 ctccaacacc agaccaccca cagcagacaa gagtatgcac atagaagcag ggagaccttt
      661 caacaaagac gttacaaatg tgagcaagtt ttcaatgaga aagttcatgt tactgagcat
      721 cagagagtcc acactggaga aaaagcttat aagcgtaggg aatatgggaa atccttgaac
      781 tctaaatact tatttgttga acaccagaga acccataatg cagaaaagcc ttatgtgtgc
      841 aatatatgtg ggaaatcatt cctccataaa caaacactcg ttgggcacca gcagagaatt
      901 cacactagag aaaggtctta tgtgtgcatc gaatgtggga aatccttgag ctccaaatac
      961 tcacttgtgg aacaccagag aacccataat ggagaaaagc cttatgtgtg caatgtatgt
     1021 gggaaatcat tccgccacaa acaaacattt gttggccatc agcagagaat ccacactgga
     1081 gagaggcctt atgtgtgtat ggaatgtggg aaatctttta ttcattccta tgaccgcatt
     1141 cgacaccaga gagttcacac tggagaaggg gcttatcagt gcagtgaatg tgggaaatcc
     1201 ttcatataca aacagtcact tcttgatcac catagaatcc acacgggaga aaggccttat
     1261 gagtgcaaag aatgtgggaa ggccttcatt cacaaaaaaa gacttcttga gcaccagaga
     1321 attcatactg gagaaaagcc ttatgtgtgc atcatatgtg ggaaatcatt tatccgctcg
     1381 tctgactaca tgcgacacca gagaattcac actggagaaa gggcttatga atgcagtgac
     1441 tgtgggaaag ccttcatctc caaacaaaca cttcttaagc atcacaaaat ccacactaga
     1501 gaaaggcctt atgaatgcag tgaatgtgga aaaggcttct accttgaggt taaacttctt
     1561 cagcaccaaa gaatccatac tagagaacaa ctttgtgagt gcaatgaatg tggaaaagtc
     1621 ttcagccacc aaaaaagact tcttgagcac cagaaagttc acactggcga aaagccctgt
     1681 gagtgcagtg aatgtgggaa atgctttaga caccgcacca gcctcattca acaccagaaa
     1741 gttcacagtg gagagaggcc ttataactgc actgcatgtg agaaggcctt tatctataaa
     1801 aacaaacttg ttgagcatca gcgaatccac accggagaaa agccgtatga atgtggtaaa
     1861 tgtgggaaag ccttcaacaa aagatattcc cttgtcaggc accagaaggt acatataaca
     1921 gaagagccct agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1981 atatcttgtg gaaaggacga cctgtctgag ccgcccccct cttg
//
