LOCUS       sequence-285759-        1346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3072
FEATURES             Location/Qualifiers
     source          1..1346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1178..1202)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1210..1251
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt tggtgatacc ccccggactg
       61 agcgaggaag aggaggctct gcagaagaaa ttcaacaagc tcaagaaaaa gaaaaaggca
      121 ttgctggctc tgaagaagca aagtagcagc agcacaacca gccaaggtgg tgtcaaacgc
      181 tcactatcag agcagcctgt catggacaca gccacagcaa cagagcaggc aaagcagctg
      241 gtgaagtcag gagccatcag tgccatcaag gctgagacca agaactcagg cttcaagcgt
      301 tctcgaaccc ttgaggggaa gttaaaggac cccgagaagg gaccagtccc cactttccag
      361 ccgttccaga ggagcatatc tgctgatgat gacctgcaag agtcatccag acgtccccag
      421 aggaaatctc tgtatgagag ctttgtgtct tctagtgatc gacttcgaga actaggacca
      481 gatggagaag aggcagaggg cccaggggct ggtgatggtc cccctcgaag ctttgactgg
      541 ggctatgaag aacgcagtgg tgcccactcc tcagcctccc ctccccgaag ccgcagccgg
      601 gaccgcagcc atgagaggaa ccgggacaga gaccgagatc gggagcggga tcgagaccgg
      661 gatcgagaca gagacagaga gcgggacagg gatcgggatc gggatcgaga tcgagaccgg
      721 gaacgggaca gggatcggga gcgggatcga gaccgagacc gagagggtcc tttccgcagg
      781 tcggattcat tccctgaacg gcgagcccct aggaaaggga atactctcta tgtatatgga
      841 gaagacatga cacccaccct tctccgtggg gccttctctc cttttggaaa catcattgac
      901 ctctccatgg acccacccag aaactgtgcc ttcgtcacct atgaaaagat ggagtcagca
      961 gatcaggccg ttgctgagct caacgggacc caggtggagt ctgtacagct caaagtcaac
     1021 atagcccgaa aacagcccat gctggatgcc gctactggca agtctgtctg gggctccctc
     1081 gctgtccaga acagccctaa gggttgccac cgggacaaga ggacccagat tgtctacagt
     1141 gatgacgtct acaaggaaaa ccttgtggat ggcttctacc caactttctt gtacaaagtg
     1201 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1261 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1321 gactcaggtg tcatgtcgtt atggga
//
