LOCUS       sequence-285831-        1802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3096
FEATURES             Location/Qualifiers
     source          1..1802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1634..1658)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1666..1707
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggaggctcc agcccagaag
       61 gctgggcagg gaggactccc caaggccgat gcccagggtg cctccggggc ccgggagaag
      121 aggcccgagg agccgaggcc cctcgaagag gaccgagctg ggagccgccc cactcaaaag
      181 ggggacctgc gtggagcggc gggcggtagg acgactcccc caggcggggg ctcccggggc
      241 tgcagcctcg gggtgagccc cggcccgggg acccggcaca gcgccgggac cagacccctc
      301 gtgcgggagc cgtgcggccc cacctcttct cagaaccctg agctggttat ccccgagggg
      361 ctgcaagccc gggagggccc ctgtaggagc ccagcgcgtg gcggggactg cagcaggaac
      421 tcctgcctgg cgtggcatcg cggggcgccc gctggggaga cgccacccgt gtgtgacccc
      481 tgtccggagc ggatccagaa ccacccccgg actcaactgt gtgaggtcca cacggactgt
      541 tggccgtgcc aaccagggac tggcgctccg acctgcccga ggaccccaaa gccgacctcc
      601 cgcgggagga accccttggt ggagcagccc cgggcttgcg cgtgcggcga ggcctttgcg
      661 tggagggccc tgcggatccc ccaggagagg ctgcaggcga cggaggagcc ccgtccgtgt
      721 gcccggtgcg ggaagcgctt ccgccccaac cagcagcagc aggcgggcaa gagtccccca
      781 gtgtgtcctg agtgcggcca aacctcgcga cctcgcccta ttgtccccga ccccccggcc
      841 cagcgactgt acgcttgcga cgagtgcggc aaggccttca cgcgcacctc cagcctgctg
      901 cagcaccagc gcatccacac gggcgagcgg ccctacgagt gcgccgagtg cggcaaggcc
      961 ttcgtgcgct gctccggcct gtaccgccac cagaagacgc actcggccga gcgccaccgg
     1021 cgtggccccg tcctggcgcg gcgcgccttc cggctggggt gcccgccctg cggggactac
     1081 agcgagcgga gtccccgacg ggggtcggga gccggggaga agccgtacga gtgcgccgac
     1141 tgcgccaagg ccttcgggct gttctcgcac ctcgtggagc accggcgcgt gcacaccggc
     1201 gagaagccct acgcgtgccc cgagtgcggc aaggccttca accagcgctc gaacctgagc
     1261 cggcaccagc gcactcacag cagcgccaag ccctacgcgt gcccactgtg cgaaaaggcc
     1321 ttcaagggcc gctcgggcct ggtgcaacac cagcgcgcgc acaccggcga gcggccctat
     1381 ggctgctccg agtgcggaaa gaccttccgc ggctgctccg agctgcgcca gcacgagcgc
     1441 ctgcactcgg gcgagaagcc ctacatctgc cgcgactgcg gcaaggcctt cgtgcgcaac
     1501 tgcagcctgg tgcgccacct gcgcacgcac acgggcgagc ggccctacgc gtgcggagat
     1561 tgcggccgcg ccttcagcca acgctccaac ctcaacgagc accggaagcg gcacgggggc
     1621 cgcgccgcgc ctttgccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1681 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1741 tcgatttctt ggctttatat atcttgtgga aaggacgact tccatcacca cagtttacct
     1801 cc
//
