LOCUS       sequence-285840-        2039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3099
FEATURES             Location/Qualifiers
     source          1..2039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1871..1895)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1903..1944
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cgcgaaggaa gaggaatgca
       61 ggcagtagtt cagatggaac cgaagattcc gatttttcta cagatctcga gcacacagac
      121 agttcagaaa gtgatggcac atcccgacga tctgctcgag tcacccgctc ctcagccagg
      181 ctaagccaga gttctcaaga ttccagtcct gttcgaaatc tgcagtcttt tggcactgag
      241 gagcctgctt actctaccag aagagtgacc cgtagtcagc agcagcctac cccagtgaca
      301 ccgaaaaaat accctcttcg gcagactcgt tcatctggtt cagaaactga gcaagtggtt
      361 gatttttcag atagagaaac taaaaataca gctgatcatg atgagtcacc gcctcgaact
      421 ccaactggaa atgcgccttc ttctgagtct gacatagaca tctccagccc caatgtatct
      481 cacgatgaga gcattgccaa ggacatgtcc ctgaaggact caggcagtga tctctctcat
      541 cgccccaagc gccgtcgctt ccatgaaagc tacaacttca atatgaagtg tcctacacca
      601 ggctgtaact ctctaggaca ccttacagga aaacatgaga gacatttctc catctcagga
      661 tgcccactgt atcataacct ctcagctgac gaatgcaagg tgagagcaca gagccgggat
      721 aagcagatag aagaaaggat gctgtctcac aggcaagatg acaacaacag gcatgcaacc
      781 aggcaccagg caccaacgga gaggcagctt cgatataagg aaaaagtggc tgaactcagg
      841 aagaaaagaa attctggact gagcaaagaa cagaaagaga aatatatgga acacagacag
      901 acctatggga acacacggga acctctttta gaaaacctga caagcgagta tgacttggat
      961 cttttccgaa gagcacaagc ccgggcttca gaggatttgg agaagttaag gctgcaaggc
     1021 caaatcacag agggaagcaa catgattaaa acaattgctt ttggccgcta tgagcttgat
     1081 acctggtacc attctccata tcctgaagaa tatgcacggc tgggacgtct ctatatgtgt
     1141 gaattctgtt taaaatatat gaagagccaa acgatactcc gccggcacat ggccaaatgt
     1201 gtgtggaaac acccacctgg tgatgagata tatcgcaaag gttcaatctc tgtgtttgaa
     1261 gtggatggca agaaaaacaa gatctactgc caaaacctgt gcctgttggc caaacttttt
     1321 ctggaccaca agacattata ttatgatgtg gagcccttcc tgttctatgt tatgacagag
     1381 gcggacaaca ctggctgtca cctgattgga tatttttcta aggaaaagaa ttcattcctc
     1441 aactacaacg tctcctgtat ccttactatg cctcagtaca tgagacaggg ctatggcaag
     1501 atgcttattg atttcagtta tttgctttcc aaagtcgaag aaaaagttgg ctccccagaa
     1561 cgtccactct cagatctggg gcttataagc tatcgcagtt actggaaaga agtacttctc
     1621 cgctacctgc ataattttca aggcaaagag atttctatca aagaaatcag tcaggagacg
     1681 gctgtgaatc ctgtggacat tgtcagcact ctgcaagccc ttcagatgct caaatactgg
     1741 aagggaaaac acctagtttt aaagagacag gacctgattg atgagtggat agccaaagag
     1801 gccaaaaggt ccaactccaa taaaaccatg gatcccagct gcttaaaatg gacccctccc
     1861 aagggcactt acccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1921 cctctcctcg gtctcgattc tacgtagtaa tgaactagcc cgtaacttga aagtatttcg
     1981 atttcttggc tttatatatc ttgtggaaag gacgatatgt tgacttccaa tgaaaggct
//
