LOCUS       sequence-285858-        1175 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3105
FEATURES             Location/Qualifiers
     source          1..1175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1007..1031)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1039..1080
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt accacaacag tagtcagaag
       61 cggcactgga ccttctccag cgaggagcag ctggcaagac tgcgggctga cgccaaccgc
      121 aaattcagat gcaaagccgt ggccaacggg aaggttcttc cgaatgatcc agtctttctt
      181 gagcctcatg aagaaatgac actctgcaaa tactatgaga aaaggttatt ggaattctgt
      241 tcggtgttta agccagcaat gccaagatct gttgtgggta cggcttgtat gtatttcaaa
      301 cgtttttatc ttaataactc agtaatggaa tatcacccca ggataataat gctcacttgt
      361 gcatttttgg cctgcaaagt agatgaattc aatgtatcta gtcctcagtt tgttggaaac
      421 ctccgggaga gtcctcttgg acaggagaag gcacttgaac agatactgga atatgaacta
      481 cttcttatac agcaacttaa tttccacctt attgtccaca atccttacag accatttgag
      541 ggcttcctca tcgacttaaa gacccgctat cccatattgg agaatccaga gattttgagg
      601 aaaacagctg atgactttct taatagaatt gcattgacgg atgcttacct tttatacacg
      661 ccttcccaaa ttgccctgac tgccatttta tctagtgcct ccagggctgg aattactatg
      721 gaaagttatt tatcagagag tctgatgctg aaagagaaca gaacttgcct gtcacagtta
      781 ctagatataa tgaaaagcat gagaaactta gtaaagaagt atgaaccacc cagatctgaa
      841 gaagttgctg ttctgaaaca gaagttggag cgatgtcatt ctgctgagct tgcacttaac
      901 gtaatcacga agaagaggaa aggctatgaa gatgatgatt acgtctcaaa gaaatccaaa
      961 catgaggagg aagagtggac tgatgacgac ctggtagaat ctctctaccc aactttcttg
     1021 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     1081 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1141 ggaaaggacg aggactcttc tttcctccac tgtga
//
