LOCUS       sequence-285906-        2645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3121
FEATURES             Location/Qualifiers
     source          1..2645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcaggc gatctttgag gccctggagg gaatggacaa tcagaccgtt
       61 ctggctgtcc agtcattatt ggatggccaa ggagcagtcc ctgatccgac aggccagagt
      121 gtcaatgcgc cccctgctat ccagccattg gatgacgagg atgtatttct ctgcgggaag
      181 tgtaagaagc aattcaactc gctgccagcg tttatgaccc acaagcggga acagtgccag
      241 gggaatgccc ccgccctggc cacagtctca ctggccacca acagcatcta cccaccttcg
      301 gcagcaccca cagcggtcca gcaggcccca actcctgcca atcgccagat ctccacatac
      361 atcacagtgc ccccgtcccc actgatccag accctggtgc aggggaacat cttggtgagc
      421 gatgatgtgc tcatgtctgc catgtcagcc ttcacatccc tggaccagcc catgccccag
      481 ggccccccac ctgtgcagag cagcctgaac atgcattccg tgcccagcta cctcacccag
      541 cctccacctc ctcctccacc tcctccacca ctgcccccac cgccaccacc tcagcctcca
      601 ccacctccac cccagagcct gggcccccct gggcgtccca accctggtgg gaacggtgtg
      661 gtggaggtgt acagtgctgc tgcgcccctg gctgggagtg gaacggtgga gatccaggca
      721 ctggggatgc agccctaccc acccctagag gtgccaaacc agtgtgtgga gcctccagta
      781 tatcccaccc ccacagtgta cagccctggc aaacagggat tcaaacccaa aggaccaaac
      841 cccgccgccc ccatgaccag cgccaccggg ggcacggtgg ccacctttga ctctccagca
      901 acgctgaaga cccgacgagc taaagctgca gggaagccaa aggctcagaa actcaagtgc
      961 tcatactgtg acaagtcatt caccaaaaac tttgacctgc agcagcacat ccgaagccac
     1021 accggtgaga agcccttcca gtgcattgca tgtggccgtg cctttgccca gaagtctaat
     1081 gttaagaaac acatgcagac ccacaaggtg tggcctccag gacacagtgg tggcaccgtg
     1141 tctcgaaact ctgtgaccgt acaggtcatg gccctgaacc ccagcaggca ggaggacgag
     1201 gaaagcacag ggttgggcca gcccctgccg ggtgcgccac agccccaggc cttgtccaca
     1261 gctggtgagg aagaggggga caagccggag tccaagcagg tggtcctcat cgacagctcc
     1321 tacctgtgcc aattctgccc cagcaaattc agcacctact tccagctcaa gtctcacatg
     1381 acccagcata agaatgagca ggtgtacaag tgtgtggtca aaagctgtgc ccagacgttc
     1441 ccaaagctcg acacatttct ggagcacatc aagagccacc aggaggagct gagctaccgc
     1501 tgccacctct gcggcaagga cttcccctcg ctgtacgacc tgggcgtgca ccagtactcc
     1561 cacagcctcc tgccacagca cagccccaag aaggacaatg ccgtctacaa gtgtgtcaaa
     1621 tgtgtcaaca aatactccac ccctgaggcc ctggagcacc acctgcagac cgccactcac
     1681 aacttcccct gcccacactg ccagaaggtg tttccttgtg aacgctacct gcggcgtcat
     1741 ctgcccaccc acggcagcgg gggcaggttc aagtgccaag tgtgcaagaa gttcttccgg
     1801 cgggagcatt atctcaaact gcatgctcac atccactcgg gtgagaagcc ctacaaatgc
     1861 tcagtgtgcg agtctgcgtt caaccgcaag gacaaactga agagacacat gttgatccac
     1921 gagcccttca agaaatacaa atgccctttc tcgacgcaca caggctgcag taaggagttc
     1981 aaccggccgg acaagctgaa ggcccacatc ctctcccact ctggcatgaa gctccacaaa
     2041 tgcgccctgt gcagcaagtc cttcagccgc cgtgcccacc tcgccgagca tcagcgcgcc
     2101 cacacgggca actacaagtt ccgctgtgct ggctgcgcca agggcttttc ccgccacaaa
     2161 tacctcaaag atcaccgctg tcgtctcggc ccccaaaagg acaaggacct gcaaacccgg
     2221 cggccccccc agaggagggc agccccccgc agttgcggca gtggtgggcg caaggtgctg
     2281 acccccttgc ctgacccgct ggggctggag gagctgaagg acacaggggc tgggctggtg
     2341 cccgaggctg tccccggcaa gccgcccttc gcagagccgg acgcggtgct gtccatcgtt
     2401 gtgggtggtg cggtgggcgc ggaaactgag ctggtggtac ctggacacgc tgaggggctg
     2461 ggctccaacc tggctctggc ggagctgcag gctggggccg agggcccatg tgccatgctc
     2521 gctgtgcccg tctacatcca ggcctccgag tagtaatgaa ctagtccgta acttgaaagt
     2581 atttcgattt cttggcttta tatatcttgt ggaaaggacg acaacgtcac tcacgccatc
     2641 ttttc
//
