LOCUS       sequence-28591-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Estall JL, Ruas JL, Choi CS, Laznik D, Badman M, Maratos-Flier E, 
            Shulman GI, Spiegelman BM
  TITLE     PGC-1alpha negatively regulates hepatic FGF21 expression by 
            modulating the heme/Rev-Erb(alpha) axis.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 29. 106(52):22510-5.
  PUBMED    20018698
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Dec 29. 106(52):22510-5."
COMMENT     Sequence Label: pLKO-1: shALAS-1
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    131..248
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        297..780
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
ORIGIN
        1 tggctttata tatcttgtgg nnggacgaaa caccggccaa gatagtagca tttgaaactc
       61 gagtttcaaa tgctactatc ttggtttttg aattctcgac ctcgagacaa atggcagtat
      121 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
      181 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
      241 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg
      301 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
      361 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      421 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      481 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      541 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      601 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      661 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      721 gcgcgnngtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ntccctcgtt
      781 g
//
