LOCUS       sequence-285921-        1733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3126
FEATURES             Location/Qualifiers
     source          1..1733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1565..1589)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1597..1638
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aagatataca aacaaatgcg
       61 gaactgaaaa gcactcagga gcagtctgtg cccgcagaaa gtgcagcggt tttgaatgac
      121 tacagtttaa ccaaatctca tgaaatggaa aatgtggaca gtggagaagg cccagccaat
      181 gaagatgaag acataggaga tgattcaatg aaagtgaaag atgaatacag tgaaagagat
      241 gagaatgttt taaagtcaga acccatggga aatgcagaag agcctgaaat cccttacagc
      301 tattcaagag aatataatga atatgaaaac attaagttgg agagacatgt tgtctcattc
      361 gatagtagca ggccaaccag tggaaagatg aactgcgatg tgtgtggatt atcctgcatc
      421 agcttcaatg tcttaatggt tcataagcga agccatactg gtgaacgccc attccagtgt
      481 aatcagtgtg gggcatcttt tactcagaaa ggtaacctcc tccgccacat taaactgcac
      541 acaggggaaa aaccttttaa gtgtcacctc tgcaactatg catgccaaag aagagatgcg
      601 ctcacggggc atcttaggac acattctgtg gagaaaccct acaaatgtga gttttgtgga
      661 aggagttaca agcagagaag ttcccttgag gagcacaagg agcgctgccg tacatttctt
      721 cagagcactg acccagggga cactgcaagt gcggaggcaa gacacatcaa agcagagatg
      781 ggaagtgaaa gagctctcgt actggacaga ttagcaagca atgtggcaaa acgaaaaagc
      841 tcaatgcctc agaaattcat tggtgagaag cgccactgct ttgatgtcaa ctataattca
      901 agttacatgt atgagaaaga gagtgagctc atacagaccc gcatgatgga ccaagccatc
      961 aataacgcca tcagctatct tggcgccgaa gccctgcgcc ccttggtcca gacaccgcct
     1021 gctcccacct cggagatggt tccagttatc agcagcatgt atcccatagc cctcacccgg
     1081 gctgagatgt caaacggtgc ccctcaagag ctggaaaaga aaagcatcca ccttccagag
     1141 aagagcgtgc cttctgagag aggcctctct cccaacaata gtggccacga ctccacggac
     1201 actgacagca accatgaaga acgccagaat cacatctatc agcaaaatca catggtcctg
     1261 tctcgggccc gcaatgggat gccacttctg aaggaggttc cccgctctta cgaactcctc
     1321 aagcccccgc ccatctgccc aagagactcc gtcaaagtga tcaacaagga aggggaggtg
     1381 atggatgtgt atcggtgtga ccactgccgc gtcctcttcc tggactatgt gatgttcacg
     1441 attcacatgg gctgccacgg cttccgtgac cctttcgagt gtaacatgtg tggatatcga
     1501 agccatgatc ggtatgagtt ctcgtctcac atagccagag gagaacacag agccctgctg
     1561 aagtgcccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1621 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1681 tggctttata tatcttgtgg aaaggacgag gatacatcct ataatgtgca tta
//
