LOCUS       sequence-285933-        1487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3130
FEATURES             Location/Qualifiers
     source          1..1487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1319..1343)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1351..1392
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtgtgaccg gaatggtggt
       61 cggcggcttc gacagtggct gatcgagcag attgacagta gcatgtatcc aggactgatt
      121 tgggagaatg aggagaagag catgttccgg atcccttgga aacacgctgg caagcaagat
      181 tataatcagg aagtggatgc ctccattttt aaggcctggg cagtttttaa agggaagttt
      241 aaagaagggg acaaagctga accagccact tggaagacga ggttacgctg tgctttgaat
      301 aagagcccag attttgagga agtgacggac cggtcccaac tggacatttc cgagccatac
      361 aaagtttacc gaattgttcc tgaggaagag caaaaatgca aactaggcgt ggcaactgct
      421 ggctgcgtga atgaagttac agagatggag tgcggtcgct ctgaaatcga cgagctgatc
      481 aaggagcctt ctgtggacga ttacatgggg atgatcaaaa ggagcccttc cccgccggag
      541 gcctgtcgga gtcagctcct tccagactgg tgggcgcagc agcccagcac aggcgtgccg
      601 ctggtgacgg ggtacaccac ctacgacgcg caccattcag cattctccca gatggtgatc
      661 agcttctact atgggggcaa gctggtgggc caggccacca ccacctgccc cgagggctgc
      721 cgcctgtccc tgagccagcc tgggctgccc ggcaccaagc tgtatgggcc cgagggcctg
      781 gagctggtgc gcttcccgcc ggccgacgcc atccccagcg agcgacagag gcaggtgacg
      841 cggaagctgt tcgggcacct ggagcgcggg gtgctgctgc acagcagccg gcagggcgtg
      901 ttcgtcaagc ggctgtgcca gggccgcgtg ttctgcagcg gcaacgccgt ggtgtgcaaa
      961 ggcaggccca acaagctgga gcgtgatgag gtggtccagg tcttcgacac cagccagttc
     1021 ttccgagagc tgcagcagtt ctataacagc cagggccggc ttcctgacgg cagggtggtg
     1081 ctgtgctttg gggaagagtt tccggatatg gcccccttgc gctccaaact cattctcgtg
     1141 cagattgagc agctgtatgt ccggcaactg gcagaagagg ctgggaagag ctgtggagcc
     1201 ggctctgtga tgcaggcccc cgaggagccg ccgccagacc aggtcttccg gatgtttcca
     1261 gatatttgtg cctcacacca gagatcattt ttcagagaaa accaacagat caccgtcttg
     1321 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     1381 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1441 tatatatctt gtggaaagga cgacagctta taaaagtatt gatgttg
//
