LOCUS       sequence-286008-        1670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3155
FEATURES             Location/Qualifiers
     source          1..1670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1502..1526)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1534..1575
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt tcgcagccgg gctggctccc
       61 ttctacgcct ccaacttcag cctctggtcg gccgcttact gctcctcggc cggcccaggc
      121 ggctgctcct tccccttgga ccccgccgcc gtcaaaaagc cctccttctg catcgcagac
      181 attctgcacg ccggcgtggg ggatctgggg gcggccccgg agggcctggc aggggcctcg
      241 gccgccgccc tcaccgcgca cttgggctcg gttcacccgc acgcctcttt ccaagcggcg
      301 gccagatccc cgcttcgacc caccccagtg gtggcgccct ccgaagtccc ggctggcttc
      361 ccgcagcggc tgtctccgct ctcagccgcc taccaccacc atcacccgca acaacaacag
      421 cagcagcaac agccgcagca gcaacagcct ccgcctccgc cccgggctgg cgccctgcag
      481 cccccggcct cggggacgcg agtggttccg aacccccacc acagtggctc tgccccggcc
      541 ccctccagca aagacctcaa atttggaatt gaccgcattt tatctgcaga atttgaccca
      601 aaagtcaaag aaggcaacac gctgagagat ctcacttccc tgctaaccgg tgggcggccc
      661 gccggggtgc acctctcagg cctgcagccc tcggccggcc agttcttcgc atctctagat
      721 cccattaacg aggcttctgc aatcctgagt cccttaaact cgaacccaag aaattcagtt
      781 cagcatcagt tccaagacac gtttccaggt ccctatgctg tgctcacgaa ggacaccatg
      841 ccgcagacgt acaaaaggaa gcgttcatgg tcgcgcgctg tgttctccaa cctgcagagg
      901 aaaggcctgg agaaaaggtt tgagattcag aagtacgtga ccaagccgga ccgaaagcag
      961 ctggcggcga tgctgggcct cacggacgca caggtgaagg tgtggttcca gaaccggcgg
     1021 atgaagtggc ggcactccaa ggaggcccag gcccaaaagg acaaggacaa ggaggctggc
     1081 gagaagccat caggtggagc cccggctgcg gatggcgagc aggacgagag gagccccagc
     1141 cgttctgaag gcgaggctga gagcgagagc agcgactccg agtccctgga catggccccc
     1201 agcgacacgg agcggactga ggggagtgag cgttctctgc accaaacaac agttattaag
     1261 gccccggtca ctggcgccct cattaccgcc agcagtgctg ggagtggtgg gagcagcggc
     1321 ggcggcggca atagtttcag cttcagcagc gccagcagtc ttagtagcag cagcaccagt
     1381 gcgggttgcg ccagcagcct tggcggcggc ggcgcctcgg agcttctccc tgcaacacag
     1441 cccacagcca gcagcgctcc caaaagcccc gagccagccc aaggcgcgct tggctgctta
     1501 tacccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1561 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1621 ctttatatat cttgtggaaa ggacgaagtt tggagaatac tccatggcac
//
