LOCUS       sequence-286014-        1751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3157
FEATURES             Location/Qualifiers
     source          1..1751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1583..1607)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1615..1656
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggcccgggc gggtttccag
       61 tctgtggctc caagcggcgg cgccggagcc tcaggagggg cgggcgcggc tgctgccttg
      121 ggcccgggcg gaactccggg gcctcctgtg cgaatgggcc cggctccggg tcaagggctg
      181 taccgctccc cgatgcccgg agcggcctat ccgagaccag gtatgttgcc aggcagccga
      241 atgacacctc agggaccttc catgggaccc cctggctatg gggggaaccc ttcagtccga
      301 cctggcctgg cccagtcagg gatggatcag tcccgcaaga gacctgcccc tcagcagatc
      361 cagcaggtcc agcagcaggc ggtccaaaat cgaaaccaca atgcaaagaa aaagaagatg
      421 gctgacaaaa ttctacctca aaggattcgt gaactggtac cagaatccca ggcctatatg
      481 gatctcttgg cttttgaaag gaaactggac cagactatca tgaggaaacg gctagatatc
      541 caagaggcct tgaaacgtcc catcaagcaa aaacggaagc tgcgaatttt catttctaac
      601 actttcaatc cggctaagtc agatgccgag gatggggaag ggacggtggc ttcctgggag
      661 cttcgggtag aaggacggct cctggaggat tcagccttgt ccaaatatga tgccactaaa
      721 caaaagagga agttctcttc cttttttaag tccttggtga ttgaactgga caaagacctg
      781 tatgggccag acaaccatct ggtagaatgg cacaggaccg ccactaccca ggagaccgat
      841 ggctttcagg tgaagcggcc gggagacgtg aatgtacggt gtactgtcct actgatgctg
      901 gattaccagc ctccccagtt taaattagac ccccgcctag ctcgactcct gggcatccat
      961 acccagactc gtccagtgat catccaagca ctgtggcaat atattaagac acataagctc
     1021 caggaccctc acgagcggga gtttgtcatc tgtgacaagt acctgcagca gatctttgag
     1081 tctcaacgta tgaagttttc agagatccct cagcggctcc atgccttgct tatgccacca
     1141 gaacctatca tcattaatca tgtcatcagt gttgacccga atgatcagaa aaagacagct
     1201 tgttatgaca ttgatgttga agtggatgac accttgaaga cccagatgaa ttcttttctg
     1261 ctgtccactg ccagccaaca ggagattgct actctagaca acaagatcca tgagacaata
     1321 gaaaccatca accagctgaa gactcagcgg gagttcatgc tgagctttgc cagagaccct
     1381 cagggtttca tcaatgactg gcttcagtcc cagtgcaggg acctcaagac aatgactgat
     1441 gtggtgggta acccagagga ggagcgccga gctgagttct acttccagcc ctgggctcag
     1501 gaggctgtgt gccgatactt ctactccaag gtgcagcaga gacgacaaga attagagcaa
     1561 gccctgggaa tccggaatac atacccaact ttcttgtaca aagtggttga tatcggtaag
     1621 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1681 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaag atacactgct
     1741 cctttagttc a
//
