LOCUS       sequence-286050-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3169
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(860..884)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             892..933
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cccaagttgc aatgtccacc
       61 ctccccgttg aagatgagga gtcctcggag agcaggatgg tggtgacatt cctcatgtca
      121 gctctcgagt ccatgtgtaa agaactggcc aagtccaaag ccgaagtggc ctgcattgca
      181 gtgtatgaaa cagacgtgtt tgtcgtcgga actgaaagag gacgtgcttt tgtcaatacc
      241 agaaaggatt ttcaaaaaga ttttgtaaaa tattgtgttg aagaagaaga aaaagctgca
      301 gagatgcata aaatgaaatc tacaacccag gcaaatcgga tgagtgtaga tgctgtagaa
      361 attgaaacac tcagaaaaac agttgaggac tatttctgct tttgctatgg gaaagcttta
      421 ggcaaatcca cagtggtacc tgtaccatat gagaagatgc tgcgagacca gtcggctgtg
      481 gtagtgcagg ggcttccgga aggtgttgcc tttaaacacc ccgagaacta tgatcttgca
      541 accctgaaat ggattctgga gaacaaagca gggatttcat tcatcattaa gagacctttt
      601 ttagagccaa agaagcatgt aggtggtcgt gtgatggtaa cagatgctga caggtcaata
      661 ctatctccag gtggaagttg tggccccatc aaagtgaaaa ctgaacccac agaagattct
      721 ggcatttccc tggaaatggc agctgtgaca gtaaaggaag aatcagaaga tcctgattat
      781 tatcaatata acattcaagg aagccaccat tcttcagagg gcaatgaagg cacagaaatg
      841 gaagtaccag cagaaggtta cccaactttc ttgtacaaag tggttgatat cggtaagcct
      901 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
      961 agtatttcga tttcttggct ttatatatct tgtggaaagg acgacgaata agaggtgccg
     1021 acgagcca
//
