LOCUS       sequence-286068-        2324 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3175
FEATURES             Location/Qualifiers
     source          1..2324
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2156..2180)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2188..2229
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc gtccaatgcg aatttttgtg
       61 aatgatgacc gccatgtgat ggcaaagcat tcttccgttt atccaacaca agaggagctg
      121 gaggcagtcc agaacatggt gtcccacacg gagcgggcgc tcaaagctgt gtccgactgg
      181 atagacgagc aggaaaaggg tagcagcgag caggcagagt ccgataacat ggatgtgccc
      241 ccagaggacg acagtaaaga aggggctggg gaacagaaga cggagcacat gaccagaacc
      301 ctgcggggag tgatgcgggt gggcctggtg gcaaagggcc tcctactcaa gggggacttg
      361 gatctggagc tggtgctgct gtgtaaggag aagcccacaa ccgccctcct ggacaaggtg
      421 gccgacaacc tggccatcca gcttgctgct gtaacagaag acaagtacga aatactgcaa
      481 tctgtcgacg atgctgcgat tgtgataaaa aacacaaaag agcctccatt gtccctgacc
      541 atccacctga catcccctgt tgtcagagaa gaaatggaga aagtattagc tggagaaacg
      601 ctatcagtca acgacccccc ggacgttctg gacaggcaga aatgccttgc tgccttggcg
      661 tccctccgac acgccaagtg gttccaggcc agagccaacg ggctgaagtc ttgtgtcatt
      721 gtgatccggg tcttgaggga cctgtgcact cgcgtgccca cctggggtcc cctccgaggc
      781 tggcctctcg agctcctgtg tgagaaatcc attggcacgg ccaacagacc gatgggtgct
      841 ggcgaggccc tgcggagagt gctggagtgc ctggcgtcgg gcatcgtgat gccagatggt
      901 tctggcattt atgacccttg tgaaaaagaa gccactgatg ctattgggca tctagacaga
      961 cagcaacggg aagatatcac acagagtgcg cagcacgcac tgcggctcgc tgccttcggc
     1021 cagctccata aagtcctagg catggaccct ctgccttcca agatgcccaa gaaaccaaag
     1081 aatgaaaacc cagtggacta caccgttcag atcccaccaa gcaccaccta tgccattacg
     1141 cccatgaaac gcccaatgga ggaggacggg gaggagaagt cgcccagcaa aaagaagaag
     1201 aagattcaga agaaagagga gaaggcagag cccccccagg ctatgaatgc cctgatgcgg
     1261 ttgaaccagc tgaagccagg gctgcagtac aagctggtgt cccagactgg gcccgtccat
     1321 gcccccatct ttaccatgtc tgtggaggtt gatggcaatt cattcgaggc ctctgggccc
     1381 tccaaaaaga cggccaagct gcacgtggcc gttaaggtgt tacaggacat gggcttgccg
     1441 acgggtgctg aaggcaggga ctcgagcaag ggggaggact cggctgagga gaccgaggcg
     1501 aagccagcag tggtggcccc tgccccagtg gtagaagctg tctccacccc tagtgcggcc
     1561 tttccctcag atgccactgc cgagaacgta aaacagcagg ggccgatcct gacaaagcac
     1621 ggcaagaacc cagtcatgga gctgaacgag aagaggcgtg ggctcaagta cgagctcatc
     1681 tccgagaccg ggggcagcca cgacaagcgc ttcgtcatgg aggtcgaagt ggatggacag
     1741 aagttccaag gtgctggttc caacaaaaag gtggcgaagg cctacgctgc tcttgctgcc
     1801 ctagaaaagc ttttccctga cacccctctc gcccttgatg ccaacaaaaa gaagagagcc
     1861 ccagtacccg tcagaggggg accgaaattt gctgctaagc cacataaccc tggcttcggc
     1921 atgggaggcc ccatgcacaa cgaagtgccc ccacccccca accttcgagg gcggggaaga
     1981 ggcgggagca tccggggacg agggcgcggg cgaggatttg gtggcgccaa ccatggaggc
     2041 tacatgaatg ccggtgctgg gtatggaagc tatgggtacg gaggcaactc tgcgacagca
     2101 ggctacagtg actttttcac agactgctac ggctatcatg attttgggtc ttcctaccca
     2161 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     2221 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     2281 atatcttgtg gaaaggacga tatgagtatt ctccaccaca gttg
//
