LOCUS       sequence-28607-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Wittmann BM, Sherk A, McDonnell DP
  TITLE     Definition of functionally important mechanistic differences among 
            selective estrogen receptor down-regulators.
  JOURNAL   Cancer Res. 2007 Oct 1. 67(19):9549-60.
  PUBMED    17909066
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2007 Oct 1. 67(19):9549-60."
COMMENT     Sequence Label: pCDNA3.1-nv5-ER beta
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             87..128
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             129..149
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    180..204
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt accatgggta agcctatccc taaccctctc ctcggtctcg
      121 attctacgga aaacctgtat tttcagggcc cggatccgtc gacgaattct gcagatatca
      181 caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgacgat ataaaaaact
      241 caccatctag ccttaattct ccttcctcct acaactgcag tcaatccatc ttacccctgg
      301 agcacggctc catatacata ccttcctcct atgtagacag ccaccatgaa tatccagcca
      361 tgacattcta tagccctgct gtgatgaatt acagcattcc cagcaatgtc actaacttgg
      421 aaggtgggcc tggtcggcag accacaagcc caaatgtgtt gtggccaaca cctgggcacc
      481 tttctccttt agtggtccat cgccagttat cacatctgta tgcggaacct caaaagagtc
      541 cctggtgtga agcaagatcg ctagaacaca ccttacctgt aaacagagag acactgaaaa
      601 ggaaggttag tgggaaccgt tgcgccagcc ctgttactgg tccaggttca aagagggatg
      661 ctcacttctg cgctgtctgc agcgattacg catcgggata tcactatgga gtctggtcgt
      721 gtgaaggatg taaggccttt tttaaaagaa gcattcaagg acataatgat tatatttgtc
      781 cagctacaaa tcagtgtaca atcgataaaa accggcgcaa gagctgccag gcctgccgac
      841 ttcggaagtg ttacgaagtg ggaatggtga agtgtggctc ccggagagag agatgtgggt
      901 accgccttgt gcggaga
//
