LOCUS       sequence-286074-        1949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3177
FEATURES             Location/Qualifiers
     source          1..1949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1781..1805)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1813..1854
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tggaggtgaa tgcaggaggt
       61 gtgattgcct atatcagttc ttccagctca gcctcaagcc ctgcctcttg tcacagtgag
      121 ggttctgaga atagtttcca gtcctcctcc tcttctgttc catcttctcc aaatagctct
      181 aattctgata ccaatggtaa tcccaagaat ggtgatctcg ccaatattga aggcatcttg
      241 aagaatgatc gaatagattg ttctatgaaa acaagcaaat cgagtgcacc tgggatgaca
      301 aaaagtcata gtggtgtgac aaaatttagt ggcatggttc tactgtgtaa agtctgtggg
      361 gatgtggcgt caggattcca ctatggagtt catgcttgcg aaggctgtaa gggtttcttt
      421 cggagaagta ttcaacaaaa catccagtac aagaagtgcc tgaagaatga aaactgttct
      481 ataatgagaa tgaataggaa cagatgtcag caatgtcgct tcaaaaagtg tctgtctgtt
      541 ggaatgtcaa gagatgctgt tcggtttggt cgtattccta agcgtgaaaa acagaggatg
      601 ctaattgaaa tgcaaagtgc aatgaagacc atgatgaaca gccagttcag tggtcacttg
      661 caaaatgaca cattagtaga acatcatgaa cagacagcct tgccagccca ggaacagctg
      721 cgacccaagc cccaactgga gcaagaaaac atcaaaagct cttctcctcc atcttctgat
      781 tttgcaaagg aagaagtgat tggcatggtg accagagctc acaaggatac ctttatgtat
      841 aatcaagagc agcaagaaaa ctcagctgag agcatgcagc cccagagagg agaacggatt
      901 cccaagaaca tggagcaata taatttaaat catgatcatt gcggcaatgg gcttagcagc
      961 cattttccct gtagtgagag ccagcagcat ctcaatggac agttcaaagg gaggaatata
     1021 atgcattacc caaatggtca tgccatttgt attgcaaatg gacattgtat gaacttctcc
     1081 aatgcttata ctcaaagagt atgtgataga gttccgatag atggattttc tcagaatgag
     1141 aacaagaata gttacctgtg caacactgga ggaagaatgc atctggtttg tccaatgagt
     1201 aagtctccat atgtggaccc tcataaatca ggacatgaaa tctgggaaga attttcgatg
     1261 agcttcactc cagcagtgaa agaagtggtg gaatttgcaa agcgtattcc tgggttcaga
     1321 gatctctctc agcatgacca ggtcaacctt ttaaaggctg ggacttttga ggttttaatg
     1381 gtacggttcg catcattatt tgatgcaaag gaacgtactg tcaccttttt aagtggaaag
     1441 aaatatagtg tggatgattt acactcaatg ggagcagggg atctgctaaa ctctatgttt
     1501 gaatttagtg agaagctaaa tgccctccaa cttagtgatg aagagatgag tttgtttaca
     1561 gctgttgtcc tggtatctgc agatcgatct ggaatagaaa acgtcaactc tgtggaggct
     1621 ttgcaggaaa ctctcattcg tgcactaagg accttaataa tgaaaaacca tccaaatgag
     1681 gcctctattt ttacaaaact gcttctaaag ttgccagatc ttcgatcttt aaacaacatg
     1741 cactctgagg agctcttggc ctttaaagtt cacccttaag acccagcttt cttgtacaaa
     1801 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1861 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1921 gacgagacgt tcagtggaat cgctatccc
//
