LOCUS       sequence-286176-        1193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3211
FEATURES             Location/Qualifiers
     source          1..1193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1025..1049)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1057..1098
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggctgccca cgttggtgct
       61 tcccggactc cccaagaggt gatggagcat tacgtgagca tgtacatcca cgggaacctg
      121 gggaaggcct gcatccccga caccatcccc aaccgcgtga cagaccacac ctgtcccagc
      181 ggaggccccc tctcacccag cctcaccacc ccgctgcccc cgctggacat ctctgtggct
      241 gagcagcagc agctgggcta catgccgctg cgggatgatt acgagatcga gtatgaccag
      301 gatgccgaga cgctcatcag cgggctctct gtcaactatg atgacgacga cgtggagatc
      361 gagctgaagc gcgcccacgt ggacatgtac gtgcggaagc tgaaagagag acagcggcgg
      421 aagaacatcg cccgtgacta caatctggtg ccagccttcc tggggaagga caagaaggag
      481 aaggaaaagg cgctgaagcg caagatcacc aaggaggaga aggagctgcg cctgaagctg
      541 aggccgctgt accagttcat gtcatgcaag gagtttgatg acctttttga aaacatgcac
      601 aaagaaaaaa tgctccgggc caagatccga gaactgcagc ggtaccggcg aaacgggatc
      661 accaagatgg aagagtcggc agagtacgag gcagcgcggc ataaacggga gaagaggaag
      721 gagaacaaaa acctagccgg ctccaaacgg ggaaaggagg acggcaaaga cagcgagttc
      781 gccgccattg agaaccttcc aggcttcgag ctcctgtcag atcgcgagaa ggtgctctgc
      841 agctctttaa acttgagtcc agcccgctac gtgactgtga agactattat aattaaagac
      901 cacctccaga agcggcaagg aatcccctcc aaaagccgcc ttcctagcta cctggacaaa
      961 gtcctaaaga aaaggatttt gaatttcctc acagaaagcg gctggatctc cagggacgcg
     1021 tctttgccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1081 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1141 tggctttata tatcttgtgg aaaggacgat cacgcaagcc actcctgctg ttc
//
