LOCUS       sequence-286179-        1898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3212
FEATURES             Location/Qualifiers
     source          1..1898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1730..1754)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1762..1803
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgatgactgc tgaatcacgg
       61 gaagccacgg gtctgtcccc acaggctgca caggagaagg atggtatcgt aatagtgaag
      121 gtggaagagg aagatgagga agaccacatg tgggggcagg attccaccct acaggacacg
      181 cctcctccag acccagagat attccgccaa cgcttcaggc gcttctgtta ccagaacact
      241 tttgggcccc gagaggctct cagtcggctg aaggaacttt gtcatcagtg gctgcggcca
      301 gaaataaaca ccaaggaaca gatcctggag cttctggtgc tagagcagtt tctttccatc
      361 ctgcccaagg agctccaggt ctggctgcag gaataccgcc ccgatagtgg agaggaggcc
      421 gtgacccttc tagaagactt ggagcttgat ttatcaggac aacaggtccc aggtcaagtt
      481 catggacctg agatgctcgc aagggggatg gtgcctctgg atccagttca ggagtcctcg
      541 agctttgacc ttcatcacga ggccacccag tcccacttca aacattcgtc tcggaaaccc
      601 cgcctcttac agtcacgagc tcttcctgct gcccacattc ctgcaccccc tcatgagggt
      661 agtcccagag accaggcgat ggcatctgca ctattcacag cggattccca ggcaatggtg
      721 aagatcgagg acatggctgt gtccctcatt ctggaggaat ggggatgtca gaatctggct
      781 cggaggaatc tcagtaggga caacaggcag gagaattatg ggagcgcatt tccccagggt
      841 ggtgaaaaca ggaatgagaa cgaggagtca acctcaaagg ctgaaacctc ggaagattca
      901 gcatcacgcg gggagacaac aggaagatcc cagaaagagt ttggagagaa acgtgaccag
      961 gagggcaaaa caggagaaag acagcagaaa aaccctgagg agaaaaccag gaaagagaaa
     1021 agagattcag ggccagctat aggaaaggac aaaaaaacca tcacaggaga gagaggtcca
     1081 agggagaagg ggaaaggatt gggaagaagc ttcagtctga gctccaactt caccacccct
     1141 gaagaagttc ccacgggaac aaagtctcac agatgtgatg aatgtggtaa atgcttcacg
     1201 agaagttcaa gccttatccg ccataaaata atccacactg gagaaaagcc ctatgaatgt
     1261 agtgagtgtg ggaaagcctt cagtcttaac tccaaccttg tcctgcatca gaggatccac
     1321 acaggagaga aacctcatga atgtaacgag tgtggcaagg ccttcagcca cagttccaat
     1381 ctcatcctcc atcagcgcat ccactctgga gagaaacctt atgaatgtaa tgagtgcggg
     1441 aaggccttca gccagagctc ggacctcacc aagcatcaga gaattcacac gggggagaaa
     1501 ccctatgaat gtagtgaatg tggaaaagct ttcaaccgaa actcatacct gattttgcat
     1561 cggagaattc acactcgaga aaagccctac aagtgcacta agtgtggcaa ggccttcacc
     1621 cgcagctcca ccctcactct gcatcacaga atccatgcca gagagagagc ctctgagtac
     1681 agcccagcct cccttgatgc atttggcgcg ttcctgaaaa gttgtgtgtt gccaactttc
     1741 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1801 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1861 tgtggaaagg acgaggctga tacacagacc gactatga
//
