LOCUS       sequence-286245-        1304 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3234
FEATURES             Location/Qualifiers
     source          1..1304
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1136..1160)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1168..1209
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaccggccg ggactcgctt
       61 tccgacgggc gcactagcag cagggcgctg gtgcctggcg gttcccctag gggctcgcgc
      121 ccccggggct tcgccatcac ggacctgctg ggcttggagg ccgagctgcc ggcgcccgct
      181 ggcccaggac agggatctgg ctgcgagggt ccggcagtcg cgccgtgccc gggcccgggg
      241 cttgacggct ccagcctggc gcgtggggcc ctaccgctgg gactcggcct cctctgtggc
      301 ttcggcacgc agccgccggc ggccgctcga gcaccctgcc tgctcctagc ggacgtgccg
      361 ttcctgccgc ccaggggccc cgagcccgct gccccgctgg ctcccagccg tccgccgcct
      421 gcgctcggcc gccagaagcg cagcgacagc gtctccacgt ccgatgagga cagccagtct
      481 gaagacagga atgacctaaa ggcatccccc accttgggca agaggaagaa gcggcggcac
      541 aggacagttt tcactgctca ccagctggaa gagttggaga aggcattcag cgaggcccac
      601 taccctgatg tgtatgcccg agaaatgctg gctgtgaaaa ctgagctccc cgaagaccgg
      661 atacaggtct ggtttcaaaa ccgcagggcc aaatggcgca agcgggagaa gcgctggggc
      721 ggcagcagcg tgatggccga gtacgggctg tacggggcca tggtgcgcca ctgcatcccg
      781 ctgccagact ccgtgctcaa ctccgccgag ggcggcctgc tgggctcctg cgcgccctgg
      841 ctcctgggga tgcataaaaa atccatgggg atgataagga agccaggaag tgaagataag
      901 ttggcaggac tctggggctc tgaccacttc aaagaaggtt ctagccagag tgagtcagga
      961 tcacagagag gctcagataa agtgagccct gagaatggct tggaagatgt ggctattgac
     1021 ctctccagct ctgcccggca ggagaccaag aaagtgcacc ctggggctgg tgctcaagga
     1081 ggctccaact ccacggcact ggaggggccc cagccaggga aggtgggagc cacattgcca
     1141 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     1201 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1261 atatcttgtg gaaaggacga tggcttttgc aaaactgact tggt
//
