LOCUS       sequence-286254-        1604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3237
FEATURES             Location/Qualifiers
     source          1..1604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1436..1460)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1468..1509
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggaggagca ggacctatcg
       61 gaggtggagc tgagccccgt gggctcggag gagccccgct gcctgtcccc ggggagcgcg
      121 ccctcgctag ggcccgacgg cggcggcggc ggatcgggcc tgcgagccag cccggggcca
      181 ggcgagctgg gcaaggtcaa gaaggagcag caggacggcg aggcggacga tgacaagttc
      241 cccgtgtgca tccgcgaggc cgtcagccag gtgctcagcg gctacgactg gacgctggtg
      301 cccatgcccg tgcgcgtcaa cggcgccagc aaaagcaagc cgcacgtcaa gcggcccatg
      361 aacgccttca tggtgtgggc tcaggcagcg cgcaggaagc tcgcggacca gtacccgcac
      421 ctgcacaacg ctgagctcag caagacgctg ggcaagctct ggaggctgct gaacgaaagt
      481 gacaagcgcc ccttcatcga ggaggctgag cggctccgta tgcagcacaa gaaagaccac
      541 ccggactaca agtaccagcc caggcggcgg aagaacggga aggccgccca gggcgaggcg
      601 gagtgccccg gtggggaggc cgagcaaggt gggaccgccg ccatccaggc ccactacaag
      661 agcgcccact tggaccaccg gcacccagga gagggctccc ccatgtcaga tgggaacccc
      721 gagcacccct caggccagag ccatggccca cccacccctc caaccacccc gaagacagag
      781 ctgcagtcgg gcaaggcaga cccgaagcgg gacgggcgct ccatggggga gggcgggaag
      841 cctcacatcg acttcggcaa cgtggacatt ggtgagatca gccacgaggt aatgtccaac
      901 atggagacct ttgatgtggc tgagttggac cagtacctgc cgcccaatgg gcacccaggc
      961 catgtgagca gctactcagc agccggctat gggctgggca gtgccctggc cgtggccagt
     1021 ggacactccg cctggatctc caagccacca ggcgtggctc tgcccacggt ctcaccacct
     1081 ggtgtggatg ccaaagccca ggtgaagaca gagaccgcgg ggccccaggg gcccccacac
     1141 tacaccgacc agccatccac ctcacagatc gcctacacct ccctcagcct gccccactat
     1201 ggctcagcct tcccctccat ctcccgcccc cagtttgact actctgacca tcagccctca
     1261 ggaccctatt atggccactc gggccaggcc tctggcctct actcggcctt ctcctatatg
     1321 gggccctcgc agcggcccct ctacacggcc atctctgacc ccagcccctc agggccccag
     1381 tcccacagcc ccacacactg ggagcagcca gtatatacga cactgtcccg gccctaccca
     1441 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     1501 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1561 atatcttgtg gaaaggacga gacacccttg taggaatcgc ggtg
//
