LOCUS       sequence-286323-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3260
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(830..854)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             862..903
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acacccccag cccagacccg
       61 ttgccttcgc ctttgcccgg ggaggaagag aaacctctgg ccttatctcc tcctgttccc
      121 cggggccgcc gaggccgtcg tcctggggga gccacctcct caaatcggac actcaaggcc
      181 tccctccctc gcaagcgggg ccgccccccc aagtcagggc aggagccccc actggtgcag
      241 gtgcaggggg tgacagcccc agtaggcagc agtggcggga gcgacctcct cctgatcgat
      301 gatcagggtg tgccctatac ggtctctgaa ggttcagcgg ctgggcctga gggctctggc
      361 cccaggaagg ccccacactt ctgcccggtg tgcctgcggg ccttccccta cctctccgac
      421 ctggagcgcc acagcatctc gcactcagag ctgaagccgc accagtgcaa ggtttgcggc
      481 aagaccttca agcgctccag ccacctgcgg cggcactgca acatccatgc cggcctgcgg
      541 cccttccgct gcccgctgtg cccccgccgc ttccgcgagg cgggcgagct ggcgcaccac
      601 caccgcgtgc actcggggga gcgcccgtac cagtgcccca tctgccggct gcgctttaca
      661 gaggccaaca cgctccggcg ccatgcgaag cgcaagcacc cggaggccat gggggtaccc
      721 ctgtgtgcac cagatccagg gtctgaaccg ccgtgggacg aggagggcat cccggccaca
      781 gcaggggccg aggaagagga ggagacagag gggaaagggg agccggcctg cccaactttc
      841 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
      901 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      961 tgtggaaagg acgaatacta aagtttccct accaacat
//
