LOCUS       sequence-286404-        1460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3287
FEATURES             Location/Qualifiers
     source          1..1460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1292..1316)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1324..1365
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtcccagga ctcggtgacc
       61 ttcgcagacg tggctgtgaa cttcaccaaa gaggagtgga ccctgctgga cccagctcag
      121 aggaatctct acagagacgt gatgctggaa aattctagga acttggcatt catagattgg
      181 gcaactccat gtaaaaccaa agacgcaacc cctcagccgg atattcttcc taaaagaaca
      241 tttcctgaag ccaacagagt gtgtctcacg agcatcagtt cccagcactc cacattaaga
      301 gaagactgga gatgccccaa aacagaggaa ccacacaggc agggggtgaa taatgtgaag
      361 ccacctgcag ttgcccctga gaaagatgaa tctcctgtta gcatttgtga agatcatgaa
      421 atgaggaacc actctaaacc tacctgcagg cttgtgcctt cacagggaga ttccataaga
      481 caatgtatcc taacacgtga ctcaagtatt ttcaagtata atcctgtctt aaacgatagt
      541 caaaaaacac atgaaaacaa cgaagacgat ggagtcttgg ggtggaacat tcagtgggtt
      601 ccgtgtggga gaaaaacaga gctgaaatca agcacatgga ctggcagtca gaacactgtg
      661 catcatatac gtgatgaaat tgatacgggg gccaacaggc accagcggaa tccatttgga
      721 aaagctttcc gtgaagacgg atcccttagg gcacacaaca ctcatggtcg agagaaaatg
      781 tatgatttta ctcagtgcga gaacacctcc agaaataact caattcacgc catgcagatg
      841 cagttgtata ccgcagagac aaacaagaag gattgtcaaa ctggggcaac ctctgccaac
      901 gctccaaatt ccggttcaca caagagtcat tgcactggag agaaaaccca taaatgcccc
      961 gaatgtggga gagccttttt ttatcagtca ttccttatga gacatatgaa aattcacact
     1021 ggagagaaac cgtatgaatg tgggaaatgt gggaaagcct ttagatattc cttacacctt
     1081 aataaacatt taagaaagca tgttgtgcag aagaagccct acgaatgtga agaatgtggg
     1141 aaagtcattc gggagtcctc aaaatataca catataagga gccacactgg agagaaaccc
     1201 tataaatgta agacatgtgg aaaagacttt gcaaagtcgt caggacttaa aaaacatctt
     1261 aagactcaca aagatgagaa gccctgtgaa ttgccaactt tcttgtacaa agtggttgat
     1321 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1381 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgacacc
     1441 tagctttgat tgtgagctag
//
