LOCUS       sequence-286407-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3288
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             286..303
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(854..878)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             886..927
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ccaccactgg ggccctgggc
       61 aactactacg tggactcgtt cctgctgggc gccgacgccg cggatgagct gagcgttggc
      121 cgctatgcgc cggggaccct gggccagcct ccccggcagg cggcgacgct ggccgagcac
      181 cccgacttca gcccgtgcag cttccagtcc aaggcgacgg tgtttggcgc ctcgtggaac
      241 ccagtgcacg cggcgggcgc caacgctgta cccgctgcgg tgtaccacca ccatcaccac
      301 cacccctacg tgcaccccca ggcgcccgtg gcggcggcgg cgccggacgg caggtacatg
      361 cgctcctggc tggagcccac gcccggtgcg ctctccttcg cgggcttgcc ctccagccgg
      421 ccttatggca ttaaacctga accgctgtcg gccagaaggg gtgactgtcc cacgcttgac
      481 actcacactt tgtccctgac tgactatgct tgtggttctc ctccagttga tagagaaaaa
      541 caacccagcg aaggcgcctt ctctgaaaac aatgctgaga atgagagcgg cggagacaag
      601 ccccccatcg atcccaataa cccagcagcc aactggcttc atgcgcgctc cactcggaaa
      661 aagcggtgcc cctatacaaa acaccagacc ctggaactgg agaaagagtt tctgttcaac
      721 atgtacctca ccagggaccg caggtacgag gtggctcgac tgctcaacct caccgagagg
      781 caggtcaaga tctggttcca gaaccgcagg atgaaaatga agaaaatcaa caaagaccga
      841 gcaaaagacg agtgcccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
      901 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
      961 tcgatttctt ggctttatat atcttgtgga aaggacgaat cttaccggca tgccgcgcac
     1021 ta
//
