LOCUS       sequence-286452-        1445 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3303
FEATURES             Location/Qualifiers
     source          1..1445
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1277..1301)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1309..1350
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc tggaaatgct agaatataat
       61 cactatcagg tgcagaccca cctcgaaaac cccaccaagt accacataca gcaagcccaa
      121 cggcagcagg taaagcagta cctttctacc actttagcaa ataaacatgc caaccaagtc
      181 ctgagcttgc catgtccaaa ccagcctggc gatcatgtca tgccaccggt gccggggagc
      241 agcgcaccca acagccccat ggctatgctt acgcttaact ccaactgtga aaaagaggga
      301 ttttataagt ttgaagagca aaacagggca gagagcgagt gcccaggcat gaacacacat
      361 tcacgagcgt cctgtatgca gatggatgat gtaatcgatg acatcattag cctagaatca
      421 agttataatg aggaaatctt gggcttgatg gatcctgctt tgcaaatggc aaatacgttg
      481 cctgtctcgg gaaacttgat tgatctttat ggaaaccaag gtctgccccc accaggcctc
      541 accatcagca actcctgtcc agccaacctt cccaacataa aaagggagct cacagagtct
      601 gaagcaagag cactggccaa agagaggcag aaaaaggaca atcacaacct gattgaacga
      661 agaagaagat ttaacataaa tgaccgcatt aaagaactag gtactttgat tcccaagtca
      721 aatgatccag acatgcgctg gaacaaggga accatcttaa aagcatccgt ggactatatc
      781 cgaaagttgc aacgagaaca gcaacgcgca aaagaacttg aaaaccgaca gaagaaactg
      841 gagcacgcca accggcattt gttgctcaga atacaggaac ttgaaatgca ggctcgagct
      901 catggacttt cccttattcc atccacgggt ctctgctctc cagatttggt gaatcggatc
      961 atcaagcaag aacccgttct tgagaactgc agccaagacc tccttcagca tcatgcagac
     1021 ctaacctgta caacaactct cgatctcacg gatggcacca tcaccttcaa caacaacctc
     1081 ggaactggga ctgaggccaa ccaagcctat agtgtcccca caaaaatggg atccaaactg
     1141 gaagacatcc tgatggacga caccctttct cccgtcggtg tcactgatcc actcctttcc
     1201 tcagtgtccc ccggagcttc caaaacaagc agccggagga gcagtatgag catggaagag
     1261 acggagcaca cttgttaccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1321 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1381 atttcgattt cttggcttta tatatcttgt ggaaaggacg attcgcaatt cttgtcattt
     1441 gacgt
//
