LOCUS       sequence-286485-        1496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3314
FEATURES             Location/Qualifiers
     source          1..1496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1328..1352)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1360..1401
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc actccaccac ccccatcagc
       61 tccctcttct ccttcaccag ccccgcagtg aagagactgc taggctggaa gcaaggagat
      121 gaagaggaaa agtgggcaga gaaggcagtg gactctctag tgaagaagtt aaagaagaag
      181 aagggagcca tggacgagct ggagagggct ctcagctgcc cggggcagcc cagcaaatgc
      241 gtcacgattc cccgctccct ggacgggcgg ctgcaggtgt cccaccgcaa gggcctgccc
      301 catgtgattt actgtcgcgt gtggcgctgg ccggatctgc agtcccacca cgagctgaag
      361 ccgctggagt gctgtgagtt cccatttggc tccaagcaga aagaagtgtg cattaaccct
      421 taccactacc gccgggtgga gactccagta ctgcctcctg tgctcgtgcc aagacacagt
      481 gaatataacc cccagctcag cctcctggcc aagttccgca gcgcctccct gcacagtgag
      541 ccactcatgc cacacaacgc cacctatcct gactctttcc agcagcctcc gtgctctgca
      601 ctccctccct cacccagcca cgcgttctcc cagtccccgt gcacggccag ctaccctcac
      661 tccccaggaa gtccttctga gccagagagt ccctatcaac actcagactt tcgaccagtt
      721 tgttacgagg agccccagca ctggtgctcg gtcgcctact atgaactgaa caaccgagtt
      781 ggggagacat tccaggcttc ctcccgaagt gtgctcatag atgggttcac cgacccttca
      841 aataacagga acagattctg tcttggactt ctttctaatg taaacagaaa ctcaacgata
      901 gaaaatacca ggagacatat aggaaagggt gtgcacttgt actacgtcgg gggagaggtg
      961 tatgccgagt gcgtgagtga cagcagcatc tttgtgcaga gccggaactg caactatcaa
     1021 cacggcttcc acccagctac cgtctgcaag atccccagcg gctgcagcct caaggtcttc
     1081 aacaaccagc tcttcgctca gctcctggcc cagtcagttc accacggctt tgaagtcgtg
     1141 tatgaactga ccaagatgtg tactatccgg atgagttttg ttaagggttg gggtgctgag
     1201 tatcatcgcc aggatgtcac cagcaccccc tgctggattg agattcatct tcatgggcca
     1261 ctgcagtggc tggacaaagt tctgactcag atgggctctc cacataaccc catttcttca
     1321 gtgtcttacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1381 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1441 tcttggcttt atatatcttg tggaaaggac gatctcgaac attagcttca caagtc
//
