LOCUS       sequence-286518-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3325
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(899..923)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             931..972
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gtggctcctt cgatcgcaag
       61 ctcagcagca tcctcaccga catctccagc tcccttagct gccatgcggg ctccaaggac
      121 tcgcctaccc tgcccgagtc ttctgtcact gacctgggct actacagcgc tccccagcac
      181 gattactact cgggccagcc ctatggccag acggtgaacc cctacaccta ccaccaccaa
      241 ttcaatctca atgggcttgc aggcacgggc gcttactcgc ccaagtcgga atatacctac
      301 ggagcctcct accggcaata cggggcgtat cgggagcagc cgctgccagc ccaggaccca
      361 gtgtcggtga aggaggagcc ggaagcagag gtgcgcatgg tgaatgggaa gcccaagaag
      421 gtccgaaagc cgcgtacaat ctactccagc taccagctgg ccgccctgca gcgccgcttc
      481 cagaaggccc agtacctggc gctgcccgag cgcgccgagc tggccgcgca gctgggcctc
      541 acgcagacac aggtgaaaat ctggttccag aaccgccgtt ccaagttcaa gaaactctac
      601 aagaacgggg aggtgccgct ggagcacagt cccaataaca gtgattccat ggcctgcaac
      661 tcaccaccat cacccgccct ctgggacacc tcttcccact ccactccggc ccctgcccgc
      721 agtcagctgc ccccgccgct cccatacagt gcctccccca gctacctgga cgaccccacc
      781 aactcctggt atcacgcaca gaacctgagt ggaccccact tacagcagca gccgcctcag
      841 ccagccaccc tgcaccatgc ctctcccggg cccccgccca accctggggc tgtgtactgc
      901 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
      961 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1021 tatatatctt gtggaaagga cgattcgaag atgcctttaa catcctc
//
