LOCUS       sequence-286563-        1496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3340
FEATURES             Location/Qualifiers
     source          1..1496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1328..1352)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1360..1401
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tggccaagac cgtggcctat
       61 ttctacgacc ccgacgtggg caacttccac tacggagctg gacaccctat gaagccccat
      121 cgcctggcat tgacccatag cctggtcctg cattacggtc tctataagaa gatgatcgtc
      181 ttcaagccat accaggcctc ccagcatgac atgtgccgct tccactccga ggactacatt
      241 gacttcctgc agagagtcag ccccaccaat atgcaaggct tcaccaagag tcttaatgcc
      301 ttcaacgtag gcgatgactg cccagtgttt cccgggctct ttgagttctg ctcgcgttac
      361 acaggcgcat ctctgcaagg agcaacccag ctgaacaaca agatctgtga tattgccatt
      421 aactgggctg gtggtctgca ccatgccaag aagtttgagg cctctggctt ctgctatgtc
      481 aacgacattg tgattggcat cctggagctg ctcaagtacc accctcgggt gctctacatt
      541 gacattgaca tccaccatgg tgacggggtt caagaagctt tctacctcac tgaccgggtc
      601 atgacggtgt ccttccacaa atacggaaat tacttcttcc ctggcacagg tgacatgtat
      661 gaagtcgggg cagagagtgg ccgctactac tgtctgaacg tgcccctgcg ggatggcatt
      721 gatgaccaga gttacaagca ccttttccag ccggttatca accaggtagt ggacttctac
      781 caacccacgt gcattgtgct ccagtgtgga gctgactctc tgggctgtga tcgattgggc
      841 tgctttaacc tcagcatccg agggcatggg gaatgcgttg aatatgtcaa gagcttcaat
      901 atccctctac tcgtgctggg tggtggtggt tatactgtcc gaaatgttgc ccgctgctgg
      961 acatatgaga catcgctgct ggtagaagag gccattagtg aggagcttcc ctatagtgaa
     1021 tacttcgagt actttgcccc agacttcaca cttcatccag atgtcagcac ccgcatcgag
     1081 aatcagaact cacgccagta tctggaccag atccgccaga caatctttga aaacctgaag
     1141 atgctgaacc atgcacctag tgtccagatt catgacgtgc ctgcagacct cctgacctat
     1201 gacaggactg atgaggctga tgcagaggag aggggtcctg aggagaacta tagcaggcca
     1261 gaggcaccca atgagttcta tgatggagac catgacaatg acaaggaaag cgatgtggag
     1321 atttagaacc cagctttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1381 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1441 tcttggcttt atatatcttg tggaaaggac gataaacgac gttatacgac actcga
//
