LOCUS       sequence-286683-        1436 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3380
FEATURES             Location/Qualifiers
     source          1..1436
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1268..1292)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1300..1341
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt tggcggctgc gctcctaaag
       61 gccaagtccc aggagctggt aacctttgag gatgtagctg tgtacttcat ccggaaggag
      121 tggaagcgtt tggaacctgc tcagagggac ctctatagag atgtgatgct ggagaattac
      181 gggaatgtgt tctcactgga tcgtgagacc aggactgaaa atgatcaaga aatttctgaa
      241 gacacaagat cacatggggt cctactggga agatttcaaa aggatatttc tcagggtctc
      301 aagtttaaag aagcctatga acgagaagtc agtctgaaaa ggccgctggg gaactcccct
      361 ggagaaagac tgaacaggaa aatgccagat tttggtcaag tgacagttga ggagaagcta
      421 acccccaggg gagagagaag cgagaaatat aatgattttg ggaacagctt cactgtgaat
      481 tccaacctta tctcacatca gagactcccc gtgggagaca gaccccataa gtgtgatgaa
      541 tgtagcaaga gctttaatcg aacttcagac cttattcaac atcagagaat ccacactggg
      601 gaaaagccct atgaatgtaa tgagtgtggg aaggccttca gccagagctc acaccttatt
      661 cagcatcaga gaatccacac tggggaaaaa ccttatgaat gtagtgattg tgggaaaacc
      721 ttcagctgta gctctgccct cattctgcat cggaggatcc acacggggga gaaaccctat
      781 gaatgtaatg agtgtgggaa gaccttcagc tggagctcca ccctcaccca ccatcagaga
      841 atccacactg gtgagaaacc ctacgcctgc aatgaatgtg ggaaggcctt cagcaggagc
      901 tcaaccctta ttcaccatca gagaatccac actggagaaa aaccctatga atgtaatgaa
      961 tgtgggaaag ccttcagcca gagctcacac ctctatcagc accagagaat ccacactgga
     1021 gagaagccct acgaatgtat ggaatgtgga ggaaagttta cctacagttc aggccttatt
     1081 cagcatcaaa gaatccacac cggggagaac ccctatgaat gtagtgagtg tgggaaagcc
     1141 ttcaggtaca gctcggctct tgttcgccat cagagaattc acactggaga gaagcctttg
     1201 aatgggatcg gcatgagcaa aagctccctc agagttacga ccgagttaaa tatcagagag
     1261 tccacgtgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1321 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1381 tcttggcttt atatatcttg tggaaaggac gaagttcaaa caaggcagtc gggatt
//
