LOCUS       sequence-286698-        1610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3385
FEATURES             Location/Qualifiers
     source          1..1610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             298..327
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             301..330
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(1442..1466)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1474..1515
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgacgatgct cctggacgga
       61 ggcccgcagt tccctgggct gggagtgggc agcttcggcg cgccgcgcca ccacgagatg
      121 cccaaccgtg agccggcagg catggggctg aatcccttcg gggactcaac ccacgccgcc
      181 gccgccgccg ccgccgccgc tgccttcaag ctgagccctg ccgcggcgca cgatctatct
      241 tcaggccaga gctcggcttt cacgccgcag ggttcgggct acgccaacgc cctgggccac
      301 catcaccacc accatcacca tcatcaccac accagccagg tgcccagcta cggtggcgct
      361 gcctctgccg ccttcaactc aacgcgcgag tttctgttcc gccagcgcag ctccgggctc
      421 agtgaggcgg cctcgggtgg cgggcagcac gggctcttcg ccggctcggc gagcagcctg
      481 catgctccag ctggcatccc cgagccccct agctacttgc tgtttcccgg gctgcatgag
      541 cagggcgctg ggcacccgtc gcccacaggg cacgtggaca acaaccaggt ccacctgggg
      601 ctgcgtgggg agctgttcgg ccgtgctgac ccataccgcc cagtggccag cccgcgcacg
      661 gacccctacg cggccggcgc tcagtttcct aactacagcc ccatgaacat gaacatggga
      721 gtgaacgtgg cggcccacca cgggcccggc gccttcttcc gttatatgcg gcagcctatc
      781 aagcaggagc tgtcgtgcaa gtggatcgac gaggctcagc tgagccggcc caagaagagc
      841 tgcgaccgga ccttcagcac catgcatgag ctggtgacac atgtcaccat ggagcatgtg
      901 gggggcccgg agcagaacaa ccacgtctgc tactgggagg agtgcccccg ggagggcaag
      961 tctttcaagg cgaagtacaa actggtcaac cacatccgag tgcacacggg cgagaagccc
     1021 ttcccatgcc ccttcccggg ctgcgggaag atctttgccc gttctgagaa cctcaagatc
     1081 cacaagagga cccacacagg tgagaaacct ttcaaatgtg aatttgaagg ctgtgacaga
     1141 cgctttgcca acagcagcga ccgtaagaag cacatgcatg tgcatacctc ggacaagccc
     1201 tatatctgca aagtgtgcga caagtcctac acgcacccga gctccctgcg caaacacatg
     1261 aaggttcatg aatctcaagg gtcagattcc tcccctgctg ccagttcagg ctatgaatct
     1321 tccactccac ccgctatagc ttctgcaaac agtaaagata ccactaaaac cccttctgca
     1381 gttcaaacta gcaccagcca caaccctgga cttcctccta attttaacga atggtacgtc
     1441 ttgccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1501 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1561 ctttatatat cttgtggaaa ggacgatcat aaccgatcaa tacgccctca
//
