LOCUS       sequence-286746-        1154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3401
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(986..1010)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1018..1059
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgtctaccag ccgtttggat
       61 gctcttccaa gagtcacatg tccaaaccat ccagatgcga ttttagtgga ggactacaga
      121 gccggtgata tgatctgtcc tgaatgtggc ttggttgtag gtgaccgggt tattgatgtg
      181 ggatctgaat ggcgaacttt cagcaatgac aaagcaacaa aagatccatc tcgagttgga
      241 gattctcaga atcctcttct gagtgatgga gatttgtcta ccatgattgg caagggcaca
      301 ggagctgcaa gttttgacga atttggcaat tctaagtacc agaatcggag aacaatgagc
      361 agttctgatc gggcaatgat gaatgcattc aaagaaatca ctaccatggc agacagaatc
      421 aatctacctc gaaatatagt tgatcgaaca aataatttat tcaagcaagt atatgaacag
      481 aagagcctga agggaagagc taatgatgct atagcttctg cttgtctcta tattgcctgt
      541 agacaagaag gggttcctag gacatttaaa gaaatatgtg ccgtatcacg aatttctaag
      601 aaagaaattg gtcggtgttt taaacttatt ttgaaagcgc tagaaaccag tgtggatttg
      661 attacaactg gggacttcat gtccaggttc tgttccaacc tttgtcttcc taaacaagta
      721 cagatggcag ctacacatat agcccgtaaa gctgtggaat tggacttggt tcctgggagg
      781 agccccatct ctgtggcagc ggcagctatt tacatggcct cacaggcatc agctgaaaag
      841 aggacccaaa aagaaattgg agatattgct ggtgttgctg atgttacaat cagacagtcc
      901 tatagactga tctatcctcg agccccagat ctgtttccta cagacttcaa atttgacacc
      961 ccagtggaca aactaccaca gctataccca actttcttgt acaaagtggt tgatatcggt
     1021 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1081 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga ccagctatcg
     1141 attcagatag tgtc
//
