LOCUS       sequence-28677-e         375 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 375)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENTR/pTER shMDC1 (441-1)
FEATURES             Location/Qualifiers
     source          1..375
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..46
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(178..277)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aagtcttgga tttgggatct tataagttcc ctatcagtga tagagatccc ccaacatgca
       61 gagattgaaa ttcaagagat ttcaatctct gcatgttgtt tttggaaaag cttggtaccg
      121 agctcggatc cactagtcca gtgtggtgga attcgcggcc gcactcgaga tatctagacc
      181 cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
      241 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgca gctctggccc
      301 gtgtctcana atctctgatg ttacattgca caagataaaa atatatcatc atgaacnata
      361 aaactgtctg cttac
//
