LOCUS       sequence-286776-        2285 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3411
FEATURES             Location/Qualifiers
     source          1..2285
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2117..2141)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2149..2190
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgacgtcggg ggcgaacggg
       61 cctggttcgg ccacggcctc ggcttccaat ccgcgcaaat ttagtgagaa gattgcgctg
      121 cagaagcagc gtcaggccga ggagacggcg gccttcgagg aggtgatgat ggacatcggc
      181 tccacccggt tacaggccca aaaactgcga ctggcataca caaggagctc tcattatggt
      241 gggtctctgc ccaatgttaa ccagattggc tctggcctgg ccgagttcca gagccccctc
      301 cactcacctt tggattcatc tcggagcact cggcaccatg ggctggtgga acgggtgcag
      361 cgagatcctc gaagaatggt gtccccactt cgccgataca cccgccacat tgacagctct
      421 ccctatagtc ctgcctactt atctcctccc ccagagtcta gctggcgaag gacgatggcc
      481 tggggcaatt tccctgcaga gaaggggcag ttgtttcgac taccatctgc acttaacagg
      541 acaagctctg actctgccct tcatacaagt gtgatgaacc ccagtcccca ggatacctac
      601 ccaggcccca cacctcccag catcctgccc agccgacgtg ggggtattct ggatggtgaa
      661 atggacccca aagtacctgc tattgaggag aacttgctag atgacaagca tttgctgaag
      721 ccatgggatg ctaagaagct atcctcatcc tcttcccgac ctcggtcctg tgaagtccct
      781 ggaattaaca tctttccatc tcctgaccag cctgccaatg tgcctgtcct cccacctgcc
      841 atgaacacgg ggggctccct acctgacctc accaacctgc actttccccc accactgccc
      901 acccccctgg accctgaaga gacagcctac cctagcctga gtgggggcaa cagtacctcc
      961 aatttgaccc acaccatgac tcacctgggc atcagcaggg gcatgggcct gggcccaggc
     1021 tatgatgcac caggacttca ttcacctctc agccacccat ccctgcagtc ctccctaagc
     1081 aatcccaacc tccaggcttc cctgagcagt cctcagcccc agcttcaggg ctcccacagc
     1141 cacccctctc tgcctgcctc ctccttggcc cgccatgtac tgcccaccac ctccctgggc
     1201 cacccctcac tcagtgctcc ggctctttcc tcctcctctt cctcctcctc cacttcatct
     1261 cctgttttgg gcgccccctc ttaccctgct tctacccctg gggcctcccc ccaccaccgc
     1321 cgtgtgcccc tcagccccct gagtttgctc gcgggcccag ccgacgccag aaggtcccaa
     1381 cagcagctgc ccaaacagtt ttcgccaaca atgtcaccca ccttgtcttc catcactcag
     1441 ggcgtccccc tggataccag taaactgtcc actgaccagc ggttaccccc atacccatac
     1501 agctccccaa gtctggttct gcctacccag ccccacaccc caaagtctct acagcagcca
     1561 gggctgccct ctcagtcttg ttcagtgcag tcctcaggtg ggcagccccc aggcaggcag
     1621 tctcattatg ggacaccgta cccacctggg cccagtgggc atgggcaaca gtcttaccac
     1681 cggccaatga gtgacttcaa cctggggaat ctggagcagt tcagcatgga gagcccatca
     1741 gccagcctgg tgctggatcc ccctggcttt tctgaagggc ctggattttt agggggtgag
     1801 gggccaatgg gtggccccca ggatccccac accttcaacc accagaactt gacccactgt
     1861 tcccgccatg gctcagggcc taacatcatc ctcacagggg actcctctcc aggtttctct
     1921 aaggagattg cagcagccct ggccggagtg cctggctttg aggtgtcagc agctggattg
     1981 gagctagggc ttgggctaga agatgagctg cgcatggagc cactgggcct ggaagggcta
     2041 aacatgctga gtgacccctg tgccctgctg cctgatcctg ctgtggagga gtcattccgc
     2101 agtgaccggc tccaatgccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     2161 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     2221 atttcgattt cttggcttta tatatcttgt ggaaaggacg aatcgtattc aaccatgaac
     2281 agtcc
//
