LOCUS       sequence-286824-        1466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3427
FEATURES             Location/Qualifiers
     source          1..1466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1298..1322)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1330..1371
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tgtattctcc catctgtctc
       61 actcaggatg aatttcaccc attcatcgag gcacttcttc cacatgtccg tgcaattgcc
      121 tatacttggt tcaacctgca ggctcgaaaa cgcaagtact ttaaaaagca tgagaagcga
      181 atgtcaaagg atgaagaaag agcagtcaaa gatgagcttc tcagtgaaaa gcctgaaatc
      241 aaacagaagt gggcatccag gctccttgcc aaactgcgca aagatattcg ccaggagtat
      301 cgagaggact ttgtgctcac cgtgactggc aagaagcacc cgtgctgtgt cttatccaat
      361 cccgaccaga agggtaagat taggagaatc gactgcctgc gacaggcaga caaagtctgg
      421 cgtctggatc tagtcatggt gatcctgttc aaaggcatcc ccttggaaag taccgatgga
      481 gagcggctca tgaaatcccc acattgcaca aacccagcac tttgtgtcca gccacatcat
      541 atcacagtat cagttaagga gcttgatttg tttttggcat actacgtgca ggagcaagat
      601 tctggacaat caggaagtcc aagccacaat gatcctgcca agaatcctcc aggttacctt
      661 gaggatagtt ttgtaaaatc tggagtcttc aatgtatcag aacttgtaag agtatccaga
      721 acgcccataa cccagggaac tggagtcaac ttcccaattg gagaaatccc aagccaacca
      781 tactatcatg acatgaactc gggggtcaat cttcagaggt ctctgtcttc tccaccaagc
      841 agcaaaagac ccaaaactat atccatagat gaaaatatgg aaccaagtcc tacaggagac
      901 ttttacccct ctccaagttc accagctgct ggaagtcgaa catggcacga aagagatcaa
      961 gatatgtctt ctccgactac tatgaagaag cctgaaaagc cattgttcag ctctgcatct
     1021 ccacaggatt cttccccaag actgagcact ttcccccagc accaccatcc cggaatacct
     1081 ggagttgcac acagtgtcat ctcaactcga actccacctc caccttcacc gttgccattt
     1141 ccaacacaag ctatccttcc tccagcccca tcgagctact tttctcatcc aacaatcaga
     1201 tatcctcccc acctgaatcc tcaggatact ctgaagaact atgtaccttc ttatgaccca
     1261 tccagtccac aaaccagcca gtcctggtac ctgggctacc caactttctt gtacaaagtg
     1321 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1381 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1441 gacccgtgca gttgagcgct agtgat
//
