LOCUS       sequence-286839-        2537 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3432
FEATURES             Location/Qualifiers
     source          1..2537
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             37..2367
                     /codon_start=1
                     /gene="Homo sapiens NR3C1"
                     /product="human glucocorticoid receptor, isoform alpha"
                     /label=glucocorticoid receptor
                     /note="acts as a transcription factor that binds to 
                     glucocorticoid response elements, and as a modulator of 
                     other transcription factors"
                     /translation="MDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASS
                     PSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGF
                     PQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDV
                     SSEQQHLKGQTGTNGGNVKLYTTDQSTFDILQDLEFSSGSPGKETNESPWRSDLLIDEN
                     CLLSPLAGEDDSFLLEGNSNEDCKPLILPDTKPKIKDNGDLVLSSPSNVTLPQVKTEKE
                     DFIELCTPGVIKQEKLGTVYCQASFPGANIIGNKMSAISVHGVSTSGGQMYHYDMNTAS
                     LSQQQDQKPIFNVIPPIPVGSENWNRCQGSGDDNLTSLGTLNFPGRTVFSNGYSSPSMR
                     PDVSSPPSSSSTATTGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCA
                     GRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATTGVSQETSENPGN
                     KTIVPATLPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSTWRIMTTLNMLGGRQVIAAVK
                     WAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSSANLLCFAPDLIINEQRMT
                     LPCMYDQCKHMLYVSSELHRLQVSYEEYLCMKTLLLLSSVPKDGLKSQELFDEIRMTYI
                     KELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLNYCFQTFLDKTMSIEFPEML
                     AEIITNQIPKYSNGNIKKLLFHQK"
     protein_bind    complement(2369..2393)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2401..2442
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg actccaaaga atcattaact
       61 cctggtagag aagaaaaccc cagcagtgtg cttgctcagg agaggggaga tgtgatggac
      121 ttctataaaa ccctaagagg aggagctact gtgaaggttt ctgcgtcttc accctcactg
      181 gctgtcgctt ctcaatcaga ctccaagcag cgaagacttt tggttgattt tccaaaaggc
      241 tcagtaagca atgcgcagca gccagatctg tccaaagcag tttcactctc aatgggactg
      301 tatatgggag agacagaaac aaaagtgatg ggaaatgacc tgggattccc acagcagggc
      361 caaatcagcc tttcctcggg ggaaacagac ttaaagcttt tggaagaaag cattgcaaac
      421 ctcaataggt cgaccagtgt tccagagaac cccaagagtt cagcatccac tgctgtgtct
      481 gctgccccca cagagaagga gtttccaaaa actcactctg atgtatcttc agaacagcaa
      541 catttgaagg gccagactgg caccaacggt ggcaatgtga aattgtatac cacagaccaa
      601 agcacctttg acattttgca ggatttggag ttttcttctg ggtccccagg taaagagacg
      661 aatgagagtc cttggagatc agacctgttg atagatgaaa actgtttgct ttctcctctg
      721 gcgggagaag acgattcatt ccttttggaa ggaaactcga atgaggactg caagcctctc
      781 attttaccgg acactaaacc caaaattaag gataatggag atctggtttt gtcaagcccc
      841 agtaatgtaa cactgcccca agtgaaaaca gaaaaagaag atttcatcga actctgcacc
      901 cctggggtaa ttaagcaaga gaaactgggc acagtttact gtcaggcaag ctttcctgga
      961 gcaaatataa ttggtaataa aatgtctgcc atttctgttc atggtgtgag tacctctgga
     1021 ggacagatgt accactatga catgaataca gcatcccttt ctcaacagca ggatcagaag
     1081 cctattttta atgtcattcc accaattccc gttggttccg aaaattggaa taggtgccaa
     1141 ggatctggag atgacaactt gacttctctg gggactctga acttccctgg tcgaacagtt
     1201 ttttctaatg gctattcaag ccccagcatg agaccagatg taagctctcc tccatccagc
     1261 tcctcaacag caacaacagg accacctccc aaactctgcc tggtgtgctc tgatgaagct
     1321 tcaggatgtc attatggagt cttaacttgt ggaagctgta aagttttctt caaaagagca
     1381 gtggaaggac agcacaatta cctatgtgct ggaaggaatg attgcatcat cgataaaatt
     1441 cgaagaaaaa actgcccagc atgccgctat cgaaaatgtc ttcaggctgg aatgaacctg
     1501 gaagctcgaa aaacaaagaa aaaaataaaa ggaattcagc aggccactac aggagtctca
     1561 caagaaacct ctgaaaatcc tggtaacaaa acaatagttc ctgcaacgtt accacaactc
     1621 acccctaccc tggtgtcact gttggaggtt attgaacctg aagtgttata tgcaggatat
     1681 gatagctctg ttccagactc aacttggagg atcatgacta cgctcaacat gttaggaggg
     1741 cggcaagtga ttgcagcagt gaaatgggca aaggcaatac caggtttcag gaacttacac
     1801 ctggatgacc aaatgaccct actgcagtac tcctggatgt ttcttatggc atttgctctg
     1861 gggtggagat catatagaca atcaagtgca aacctgctgt gttttgctcc tgatctgatt
     1921 attaatgagc agagaatgac tctaccctgc atgtacgacc aatgtaaaca catgctgtat
     1981 gtttcctctg agttacacag gcttcaggta tcttatgaag agtatctctg tatgaaaacc
     2041 ttactgcttc tctcttcagt tcctaaggac ggtctgaaga gccaagagct atttgatgaa
     2101 attagaatga cctacatcaa agagctagga aaagccattg tcaagaggga aggaaactcc
     2161 agccagaact ggcagcggtt ttatcaactg acaaaactct tggattctat gcatgaagtg
     2221 gttgaaaatc tccttaacta ttgcttccaa acatttttgg ataagaccat gagtattgaa
     2281 ttccccgaga tgttagctga aatcatcacc aatcagatac caaaatattc aaatggaaat
     2341 atcaaaaaac ttctgtttca tcaaaagtgc ccaactttct tgtacaaagt ggttgatatc
     2401 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     2461 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgactcagcc
     2521 acgccaaaac gtattaa
//
