LOCUS       sequence-286899-        1895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3452
FEATURES             Location/Qualifiers
     source          1..1895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1727..1751)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1759..1800
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcggcggc gtggatggct
       61 ccggcgcagg agtctgtgac cttcgaggat gtggctgtga catttaccca ggaggagtgg
      121 gggcagttgg acgtgaccca gagggccttg tacgtggagg tgatgctgga gacctgtggg
      181 cttctggtcg cactgggtga cagcacaaaa cctgagaccg tagagcctat cccttctcat
      241 ctggccttgc ctgaggaagt ctcactccag gaacagctgg cacagggagt cccaagatac
      301 tcctatttgg ggcaggccat ggatcaagat gggccatctg aaatgcagga atactttttg
      361 agaccaggga cagacccaca gagtgagaaa ctccatggga aaatgagcct tgaacacgaa
      421 ggtttggcga cagctgatgg tatttgttca atgatgatac agaaccaagt ctcaccagaa
      481 gatgctctct atggatttga ctcatatgga ccagttacag attccttgat tcatgaaggg
      541 gaaaattcct ataaattcga ggaaatgttt aatgagaatt gcttccttgt tcagcatgag
      601 cagattctcc ctcgtgtgaa gccctatgat tgcccagaat gtgggaaagc cttcggcaag
      661 agcaaacacc tccttcagca tcacatcatc catactgggg agaagcccta taaatgcctg
      721 gagtgtggga aagacttcaa ccgcaggtca cacctcacac ggcaccagcg gactcacaat
      781 ggagataagc cctttgtgtg cagtgaatgt ggaaggacct tcaatcgcgg gtcgcacctt
      841 acacggcacc agcgggttca cagtggagag aagccttttg tgtgcaatga atgtggaaag
      901 gcctttacct accgctccaa ttttgtcttg cataacaaga gccacaatga gaagaaaccc
      961 ttcgcatgca gcgaatgtgg aaaaggcttt tatgagagta cagccctcat tcaacacttc
     1021 attatccata ctggggagag gccctttaag tgccttgagt gtgggaaggc cttcaactgc
     1081 aggtcacacc tcaagcagca tgagcggatt cacactggtg agaagccctt tgtgtgcagt
     1141 caatgtggaa aggccttcac tcactattcc acctatgtcc tgcatgaaag agcccacact
     1201 ggagaaaagc cttttgagtg caaagaatgt gggaaagcct ttagcattcg aaaagacctc
     1261 attcgacact tcaacatcca cactggagag aagccctatg agtgtttaca gtgtggaaag
     1321 gcttttaccc gcatgtcagg gctcacaagg caccagtgga ttcatactgg agagaagccg
     1381 tatgtatgca tccaatgtgg gaaagccttt tgtcggacca caaacctgat tcgacacttt
     1441 agcatccaca ctggagagaa gccctatgaa tgcgtggagt gcgggaaggc cttcaaccgc
     1501 aggtcacccc tcacaaggca ccagcggatt cacactgcag agaagtccca cgaacccatc
     1561 cagagtggga acgtttcttg tgagagcaca gatctcattc aacactccat catccacact
     1621 gagagtagcc cagtgagtgc agtgaatatg gaaacgcctt caattgccgc tcattcctcc
     1681 tcactcgaca tcaacggatt catagtggaa gaaaccctac cattgttgcc aactttcttg
     1741 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     1801 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1861 ggaaaggacg agccgatata ttggtttaag tgcgg
//
