LOCUS       sequence-286905-        2050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3454
FEATURES             Location/Qualifiers
     source          1..2050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1883..1907)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1915..1956
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggagcgggc gctagagccc
       61 gaggcggagg cggaggctga ggcgggcgcg ggcggggagg cagcagccga ggagggcgca
      121 gcgggccgaa aggcgcgggg ccggccgcga ctcacggagt cggaccgggc ccggcgacgg
      181 ctcgagtccc ggaagaagta cgacgtgcgg cgcgtgtacc tgggcgaggc gcacgggccc
      241 tgggtggacc tgcggcgccg cagcggctgg agcgacgcca agctcgccgc ctacctcatc
      301 tctctggagc gcggccagcg gagcggccgc cacgggaagc cttgggagca ggtccccaaa
      361 aagccaaagc ggaagaaaag gcggcgacgc aacgtgaact gcctgaagaa cgtggtgatc
      421 tggtacgagg accacaagca ccgctgcccg tacgagccgc acctggcgga gctagacccc
      481 acttttggcc tgtacaccac ggccgtgtgg cagtgcgaag ctggccaccg ctacttccag
      541 gacctgcatt cgcccctgaa gcccctcagc gactcagacc ctgacagtga caaagtgggc
      601 aatgggctgg tggctggcag ctctgactca tccagctctg gctctgcctc tgactctgag
      661 gagtctcctg agggccagcc ggtcaaggct gcggcagcgg cagcggcagc gacgcccacc
      721 agcccggtgg gcagcagcgg gctcatcact caggagggcg tgcacattcc ctttgacgtc
      781 caccacgtgg aaagcctggc cgagcagggt accccgctgt gctccaaccc agcaggcaat
      841 gggcctgaag ccctggagac agtggtgtgc gtgccggtgc ctgtgcaagt gggtgcgggc
      901 cccagcgccc tctttgagaa cgtgccccag gaggccctgg gtgaggtggt ggccagctgc
      961 cccatgccag gcatggtgcc cggctcacag gtgatcatca ttgcgggccc tggttacgac
     1021 gctctcacgg ccgagggcat tcacctcaac atggcagcag gcagcggtgt ccccggcagt
     1081 ggactgggcg aggaggtgcc ctgtgccatg atggagggtg tggcagccta cacccagaca
     1141 gagcccgagg gtagccagcc tagcaccatg gacgccaccg cagtagcagg catcgagacc
     1201 aagaaagaga aggaggacct gtgcttgcta aagaaggagg agaaggagga gccagtagcc
     1261 ccggagctgg caacaacggt gcctgagagc gcagagcctg aggcagaggc ggacggggag
     1321 gagctggacg gcagcgacat gtcagccatc atctatgaaa tccccaagga gcctgagaag
     1381 aggcggcgga gcaagcggtc gcgggtgatg gatgctgacg gcctgctcga gatgttccac
     1441 tgcccatacg agggctgcag ccaagtctac gtggccctca gcagcttcca gaaccacgtc
     1501 aatcttgtgc atcggaaagg aaagaccaaa gtgtgccctc atcctggctg tggcaagaag
     1561 ttctatttat ccaaccacct gcggcggcac atgatcatcc attcaggtgt ccgtgaattc
     1621 acctgcgaga cctgcggcaa gtccttcaag aggaagaacc acctggaggt acatcggcgc
     1681 acccacaccg gcgagacccc cctgcagtgc gagatctgtg gctaccagtg ccggcagcgc
     1741 gcgtcgctca actggcacat gaagaagcac actgcggagg tgcagtacaa cttcacgtgc
     1801 gatcgctgcg ggaagcgctt cgagaagctg gacagcgtca agttccacac gctcaaaagc
     1861 cacccggatc acaagcccac ctgcccaact ttcttgtaca aagtggttga tatcggtaag
     1921 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1981 gaaatatttc gatttcttgg ctttatatat cttgtggaaa ggacgagtat gtgtgggcgc
     2041 tcacgcaacg
//
