LOCUS       sequence-286941-        1919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3466
FEATURES             Location/Qualifiers
     source          1..1919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1751..1775)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1783..1824
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaaggagaa gtccaagaat
       61 gcggccaaga ccaggaggga gaaggaaaat ggcgagtttt acgagcttgc caagctgctc
      121 ccgctgccgt cggccatcac ttcgcagctg gacaaagcgt ccatcatccg cctcaccacg
      181 agctacctga agatgcgcgc cgtcttcccc gaaggtttag gagacgcgtg gggacagccg
      241 agccgcgccg ggcccctgga cggcgtcgcc aaggagctgg gatcgcactt gctgcagact
      301 ttggatggat ttgtttttgt ggtagcatct gatggcaaaa tcatgtatat atccgagacc
      361 gcttctgtcc atttaggctt atcccaggtg gagctcacgg gcaacagtat ttatgaatac
      421 atccatcctt ctgaccacga tgagatgacc gctgtcctca cggcccacca gccgctgcac
      481 caccacctgc tccaagagta tgagatagag aggtcgttct ttcttcgaat gaaatgtgtc
      541 ttggcgaaaa ggaacgcggg cctgacctgc agcggataca aggtcatcca ctgcagtggc
      601 tacttgaaga tcaggcagta tatgctggac atgtccctgt acgactcctg ctaccagatt
      661 gtggggctgg tggccgtggg ccagtcgctg ccacccagtg ccatcaccga gatcaagctg
      721 tacagtaaca tgttcatgtt cagggccagc cttgacctga agctgatatt cctggattcc
      781 agggtgaccg aggtgacggg gtacgagccg caggacctga tcgagaagac cctataccat
      841 cacgtgcacg gctgcgacgt gttccacctc cgctacgcac accacctcct gttggtgaag
      901 ggccaggtca ccaccaagta ctaccggctg ctgtccaagc ggggcggctg ggtgtgggtg
      961 cagagctacg ccaccgtggt gcacaacagc cgctcgtccc ggccccactg catcgtgagt
     1021 gtcaattatg tactcacgga gattgaatac aaggaacttc agctgtccct ggagcaggtg
     1081 tccactgcca agtcccagga ctcctggagg accgccttgt ctacctcaca agaaactagg
     1141 aaattagtga aacccaaaaa taccaagatg aagacaaagc tgagaacaaa cccttacccc
     1201 ccacagcaat acagctcgtt ccaaatggac aaactggaat gcggccagct cggaaactgg
     1261 agagccagtc cccctgcaag cgctgctgct cctccagaac tgcagcccca ctcagaaagc
     1321 agtgaccttc tgtacacgcc atcctacagc ctgcccttct cctaccatta cggacacttc
     1381 cctctggact ctcacgtctt cagcagcaaa aagccaatgt tgccggccaa gttcgggcag
     1441 ccccaaggat ccccttgtga ggtggcacgc tttttcctga gcacactgcc agccagcggt
     1501 gaatgccagt ggcattatgc caacccccta gtgcctagca gctcgtctcc agctaaaaat
     1561 cctccagagc caccggcgaa cactgctagg cacagcctgg tgccaagcta cgaaggtggg
     1621 tcaggtctgc tcgtggggaa ggtgggagga ctgcgcacgg ccgggagccg aagcagccat
     1681 ggcggtgggt ggcagatgga gacagaaccc tcacgctttg ggcaaacttg ccctctttct
     1741 gcttctaagt tgccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1801 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1861 atttcttggc tttatatatc ttgtggaaag gacgactccg ttccattctt gtagtaact
//
