LOCUS       sequence-286947-        1514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3468
FEATURES             Location/Qualifiers
     source          1..1514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1346..1370)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1378..1419
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tggctacaca gacattaagt
       61 atagacagct atcaagatgg gcaacagatg caagtagtaa cagagttaaa gacagaacaa
      121 gatccaaact gctctgaacc cgatgcagaa ggagtgagcc ctccccctgt ggagtctcag
      181 accccgatgg atgtggacaa gcaggccatt tataggcatc cactatttcc attattagct
      241 ttgttgtttg aaaaatgtga acaatctaca cagggctctg aaggcacaac ttctgccagt
      301 tttgatgtag acatcgaaaa ttttgtaaga aagcaagaga aggaagggaa acctttcttt
      361 tgtgaagatc cagaaaccga taatttaatg gtaaaagcaa tccaggtttt gcgcattcat
      421 cttcttgagc tggaaaaggt taacgaactc tgcaaagatt tctgcagtcg atacattgct
      481 tgtctgaaaa caaaaatgaa cagtgaaact ctgttgagtg gagagcctgg aagcccgtac
      541 tcaccagtgc agtcccagca gattcaaagt gccatcacag gcaccatcag ccctcaggga
      601 attgtggtgc cggcgtccgc gctgcagcag ggaaacgtag ccatggcgac ggtggcaggt
      661 ggcacagtgt atcagcctgt cacggtcgtc actccccaag gccaagtggt cacacagaca
      721 ttgtcgcctg ggacaattag gatccagaac tcccagcttc agttacagtt aaaccaagat
      781 ctcagcatct tgcatcaaga tgatggttca tctaagaaca agaggggcgt cctgccaaag
      841 catgccacga acgtgatgcg gtcctggctc ttccagcaca tcgggcatcc ctacccaaca
      901 gaggatgaga aaaaacagat tgctgctcag acaaatttga cactactcca agtcaacaac
      961 tggttcatca atgccagaag acgaattctt cagccaatgt tggattcaag ttgttcagag
     1021 acccccaaaa caaagaaaaa aactgctcag aaccggccag ttcagaggtt ttggcctgat
     1081 tctattgcat caggagtcgc acagccaccg ccgagcgagc tcaccatgtc ggaaggagct
     1141 gttgtcacca tcaccacgcc cgtgaacatg aacgtggaca gccttcagtc tctgtcctcg
     1201 gacggggcca ccctggcggt gcagcaggtc atgatggcag ggcagagcga ggacgagtct
     1261 gtggacagca cagaggagga tgcgggtgcc ctggcccctg cccacatcag cgggctggtc
     1321 ttggagaaca gtgactccct gcagtaccca actttcttgt acaaagtggt tgatatcggt
     1381 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1441 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga attctgagat
     1501 cgggaagact gaca
//
