LOCUS       sequence-286986-        1478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3481
FEATURES             Location/Qualifiers
     source          1..1478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1310..1334)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1342..1383
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgtccatgcc cccgacgccc
       61 gaggcccagg gaccaatact gtttgaggat ctggctgtgt atttttctca agaggagtgc
      121 gtgactctgc accctgccca gaggtccctc agcaaagatg gtacaaaaga gtctttggag
      181 gatgcggctt tgatgggaga ggaaggcaag cctgagatta atcagcagtt aagcctagag
      241 tctatggaac ttgacgagct tgccttagaa aagtacccca ttgctgcacc ccttgtccct
      301 tacccagaaa aatcctctga ggatggagtt ggaaaccctg aagcgaaaat attaagtgga
      361 actcccactt acaagagaag ggtcatcagc cttttagtta ccattgaaaa ccacacccca
      421 ttagtagaac tctctgaata tttaggaacc aacacacttt ctgaaattct tgattctccc
      481 tgggaaggag ccaaaaatgt gtacaaatgt cctgagtgtg accaaaactt cagcgatcat
      541 tcatacctag ttttgcatca gaaaattcat tcaggagaga aaaaacataa atgtggtgac
      601 tgtggaaaga tcttcaatca tagagccaac ctgaggacac acaggagaat ccatactggt
      661 gagaaacctt ataagtgtgc caagtgcagt gccagctttc gccagcactc tcatctatcc
      721 cgacacatga atagccacgt aaaggagaag ccctatacat gtagcatatg tggtagaggt
      781 tttatgtggc tcccaggatt ggcacagcat cagaaaagcc acagtgctga aaacacctac
      841 gaatctacta actgtgataa acattttaat gagaaaccaa atcttgcttt gcctgaggaa
      901 acattcgtat caggccccca gtaccagcac actaagtgca tgaagagctt caggcagtcc
      961 ttatatcctg ccctttccga gaagagccac gacgaggact ctgaacgctg cagcgatgat
     1021 ggggacaatt tcttctcatt ctcaaaattc aagcccttac agtgtcctga ctgtgacatg
     1081 acctttcctt gtttctctga gcttatttcc catcagaaca ttcatacaga ggaaaggccc
     1141 cataagtgca aaacatgcga ggaaagtttt gctttggact cagaacttgc atgccaccag
     1201 aagagccaca tgctagcgga accttttaaa tgtaccgtgt gtgggaaaac tttcaagtcg
     1261 aatttgcatc tcattactca taagcgaact cacataaaaa acaccacgta cccaactttc
     1321 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     1381 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1441 tgtggaaagg acgattctaa gcgctcgaaa aataaatt
//
